Objective To investigate the relationship between B?lymphocyte?induced maturation protein?1 (Blimp?1) and the proliferation, apoptosis, and invasion of the hepatocellular carcinoma cell line SMMC?7721. Methods The experiment included a control group, shRNA?NC group, and shRNA?Blimp1 group. The expression of the Blimp?1gene was silenced in hepatocellular carcinoma cells. Real?time quantitative PCR (RT?PCR) and Western blotting were used to detect Blimp?1 mRNA and protein expression. Proliferation and apoptosis were measured by the CCK?8 method and flow cytometry. The Transwell method was used to observe the invasion ability of cells. The protein levels of IFN?γ and IL?2 were analyzed by ELISA. Results Compared with the levels in the control group, the expression of Blimp?1 mRNA and protein in the shRNA?Blimp1 group was significantly decreased ( P < 0. 05). Silencing Blimp?1 had significant effects on inhibiting SMMC?7721 cell proliferation ( P < 0. 05), promoting cell apoptosis ( P < 0. 05), significantly reducing the invasive ability of cells ( P < 0. 05), and significantly increasing the level of IFN?γ protein ( P < 0. 05), but had nosignificant effect on IL?2 protein level. Conclusions Silencing Blimp?1 can promote the expression of IFN?γ protein and inhibit the proliferation, invasion, and apoptosis of hepatoma cells.