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邓浩,彭启旺,李伟.沉默Blimp?1 促进IFN?γ 表达抑制肝癌细胞的作用研究[J].中国比较医学杂志,2018,28(9):61~65.
沉默Blimp?1 促进IFN?γ 表达抑制肝癌细胞的作用研究
Silencing Blimp?1 inhibits hepatoma cells by promoting IFN?γ expression
投稿时间:2018-03-16  
DOI:10.3969/j. issn. 1671 -7856. 2018. 09. 010
中文关键词:  Blimp?1  IFN?γ  肝癌  增殖
英文关键词:Blimp?1  IFN?γ  liver cancer  proliferation
基金项目:
作者单位E-mail
邓浩 武汉市红十字会医院普通外科,武汉 430021 cdm197001@163. com 
彭启旺 武汉市红十字会医院普通外科,武汉 430021  
李伟 武汉市红十字会医院普通外科,武汉 430021  
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中文摘要:
      目的 探究B 淋巴细胞诱导成熟蛋白?1(B?lymphocyte?induced maturation protein?1,Blimp?1)与肝癌细胞SMMC?7721 增殖?凋亡?侵袭的关系以及作用机制?方法 实验分为对照组?shRNA?NC 组?shRNA?Blimp1 组;沉默肝癌细胞中Blimp?1 基因的表达,实时荧光定量PCR(RT?PCR)和蛋白质印迹法(Western blot)检测Blimp?1mRNA 和蛋白表达,CCK?8 法观察细胞的增殖,流式细胞仪检测细胞的凋亡,Transwell 法观察细胞的侵袭能力,酶联免疫吸附测定法(ELISA) 检测γ?干扰素(IFN?γ)?白细胞介素?2(IL?2) 的蛋白水平?结果 与对照组相比, shRNA?Blimp1 组细胞中Blimp?1 mRNA 和蛋白的表达量均显著降低( P < 0. 05)?沉默Blimp?1 表达显著抑制SMMC?7721 细胞增殖( P < 0. 05),促进其凋亡( P < 0. 05),显著降低细胞的侵袭能力( P < 0. 05),显著上调IFN?γ蛋白水平( P < 0. 05),但对IL?2 蛋白水平明显影响?结论 沉默Blimp?1 可促进IFN?γ 蛋白表达,从而抑制肝癌细胞增殖?侵袭及促进凋亡?
英文摘要:
      Objective To investigate the relationship between B?lymphocyte?induced maturation protein?1 (Blimp?1) and the proliferation, apoptosis, and invasion of the hepatocellular carcinoma cell line SMMC?7721. Methods The experiment included a control group, shRNA?NC group, and shRNA?Blimp1 group. The expression of the Blimp?1gene was silenced in hepatocellular carcinoma cells. Real?time quantitative PCR (RT?PCR) and Western blotting were used to detect Blimp?1 mRNA and protein expression. Proliferation and apoptosis were measured by the CCK?8 method and flow cytometry. The Transwell method was used to observe the invasion ability of cells. The protein levels of IFN?γ and IL?2 were analyzed by ELISA. Results Compared with the levels in the control group, the expression of Blimp?1 mRNA and protein in the shRNA?Blimp1 group was significantly decreased ( P < 0. 05). Silencing Blimp?1 had significant effects on inhibiting SMMC?7721 cell proliferation ( P < 0. 05), promoting cell apoptosis ( P < 0. 05), significantly reducing the invasive ability of cells ( P < 0. 05), and significantly increasing the level of IFN?γ protein ( P < 0. 05), but had nosignificant effect on IL?2 protein level. Conclusions Silencing Blimp?1 can promote the expression of IFN?γ protein and inhibit the proliferation, invasion, and apoptosis of hepatoma cells.
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