基于肝门静脉注射肿瘤细胞的新型进展期肝脏肿瘤动物模型的建立
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(解放军第三〇二医院临床研究管理中心,北京 100039)

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R-33

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Constructing an intrahepatic model of advanced liver cancer by injecting cancer cells through the hepatic portal vein
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(Research Center for Clinical and Translational Medicine, 302 Military Hospital of China, Beijing 100039, China)

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    摘要:

    目的 利用肝门静脉注射肿瘤细胞的方法,建立新型进展期肝脏肿瘤(肝细胞癌?结直肠癌肝转移?乳腺癌肝转移以及非小细胞肺癌肝转移)的动物模型?方法 培养获得人肝细胞癌MHCC97?H?人结直肠癌SW480?人非小细胞肺癌A549 以及人三阴性乳腺癌MDA?MB?231 等细胞系,将这些恶性肿瘤细胞经由BALB/ c 裸小鼠肝门静脉注入肝脏,经3 ~4 周生长后进行PET/ CT 检测:尾静脉注射约200 μCi 核素探针18 F?FDG,30 ~40 min后对BALB/ c 裸小鼠进行PET/ CT 检测?在此基础上,收集动物获取肝脏进行拍照,确定BALB/ c 裸小鼠肝脏上的肿瘤病灶?结果 MHCC97?H?SW480?A549 以及MDA?MB?231 等细胞均能够在BALB/ c 裸小鼠肝脏形成多发?弥散的肿瘤病灶?肿瘤细胞在裸小鼠肝脏原位的生长可以使用PET/ CT 检测?SW480?MDA?MB?231 以及A549 细胞形成的病灶,其PET/ CT 检测信号弱于MHCC97?H 细胞?结论 成功获得了新型进展期肝脏肿瘤动物模型,为进展期肝脏肿瘤相关药物筛选奠定了坚实基础?

    Abstract:

    Objective To construct an intrahepatic model of advanced liver cancer (hepatocellular carcinoma, colorectal cancer liver metastasis, breast cancer liver metastasis, non?small cell lung cancer liver metastasis) by injecting cancer cells through the hepatic portal vein. Methods MHCC97?H, a highly aggressive cell line of hepatocellular carcinoma; SW480, a metastatic colorectal cancer cell line; MDA?MB?231, a human triple?negative breast cancer cell line; or A549, a non?small cell lung cancer cell line, was cultured and seeded into the liver of BALB/ c nude mice by injecting cancer cells through the hepatic portal vein. After 3 - 4 weeks of growth, mice were analyzed by PET/ CT screening. Mice were injected with approximately 200 μCi nuclide probe18 F?FDG through the tail vein. The BALB/ c nude mice were analyzed by PET/ CT screening after 30 -40 min. The mice were then collected, and their livers were obtained and photographed to determine the presence of tumor nodules on the liver of BALB/ c nude mice. Results MHCC97?H, A549, SW480, or MDA?MB?231 cells grew and multiple diffuse tumor nodules formed in the nude mouse livers. Nodules in the nude mouse livers formed by cancer cells could be identified by PET/ CT screening; the PET/ CT intensity of nodules formed after A549, SW480, or MDA?MB?231 cell injection was weaker than that of MHCC97?H cells. Conclusions An intrahepatic tumor animal model of advanced liver cancer has been successfully constructed by injecting cancer cells through the hepatic portal vein. This is a promising model for research on liver cancer or antitumor drugs.

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柴燕涛,姜棋予,冯帆,孙慧伟,李晓娟,杨锐创,王志杰,李瑞生,侯俊.基于肝门静脉注射肿瘤细胞的新型进展期肝脏肿瘤动物模型的建立[J].中国比较医学杂志,2018,28(9):55~60.

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  • 收稿日期:2018-03-31
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  • 在线发布日期: 2018-10-08
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