Objective To screen the polymorphisms of the promoter regulatory region of the PPARγ2 gene in Wuzhishan minipigs. Methods Five pairs of primers were designed based on the promoter regulatory region of the PPARγ2 gene in pigs. Then, the polymorphisms of 57 outbred Wuzhishan minipigs were detected; moreover, the promoters, transcription factor?binding sites, and secondary structures of RNA and CpG islands of the regulatory region were predicted and analyzed by various types of bioinformatic software. Results Nine single?nucleotide polymorphisms (SNPs) were found, which were - 1595T/ C, - 1534G/ A, - 1262C/ A, - 1220C/ A, - 1017A/ G, - 963A/ G, -955G/ A, -866A/ G, and - 333G/ A. SNPs were not located in the region of promoters that were identified by the software, but the -333G/ A mutation was located in the core promoter region ( - 656 to - 23 bp). Transcription factor?binding sites were altered near the mutational locus. The minimum free energy of secondary structures of RNA was changed and the structure of RNA was significantly altered. CpG islands were not found in the target sequence. Conclusions The screened SNPs of the promoter region of the PPARγ2 gene in Wuzhishan minipigs may affect regulation of the expression of this gene, which lays the foundation for further study of its function.