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郭玉倩,角建林,袁鑫,吴超,王利梅,郑红.树鼩大肠内容物细菌的群落多样性分析[J].中国比较医学杂志,2018,28(9):7~13.
树鼩大肠内容物细菌的群落多样性分析
Diversity analysis of the tree shrew hindgut microbiota by Illumina PE250 sequencing technology
投稿时间:2018-05-28  
DOI:10.3969/j. issn. 1671 -7856. 2018. 09. 002
中文关键词:  树鼩  大肠细菌  Illumina PE250  多样性
英文关键词:tree shrew  hindgut microbiota  Illumina PE250  diversity
基金项目:国家自然科学基金资助项目(编号:81460647);云南省应用基础研究计划项目(编号:2017FE468?014,2017FE468016);云南省教育厅科学研究基金项目(编号:2018Y048)?
作者单位E-mail
郭玉倩 昆明医科大学实验动物学部,昆明 650500 1461553155@ qq. com 
角建林 昆明医科大学技术转移中心,昆明 650031  
袁鑫 昆明医科大学第一附属医院,昆明 650032  
吴超 昆明医科大学实验动物学部,昆明 650500  
王利梅 昆明医科大学实验动物学部,昆明 650500  
郑红 昆明医科大学实验动物学部,昆明 650500 847255170@ qq. com 
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中文摘要:
      目的 应用高通量测序技术分析树鼩大肠细菌的结构与组成?方法 采集3 只雄性树鼩大肠内容物,用细菌16SrDNA 通用引物扩增V3 ~ V4 区,采用Illumina PE250 平台测序?结果 共获得160 157 条有效序列,聚类后得437 个运算分类单位(operational taxonomic units,OTUs)?树鼩大肠中的细菌有9 个门?19 个纲?37 个目?68 个科?137 个属和194 个种?其中,①厚壁菌门和变形菌门的丰度最高,分别为63. 30% 和28. 52%;②优势纲是芽孢杆菌纲和γ?变形菌纲(51. 46%?28. 02%);③乳杆菌目和肠杆菌目的丰度最高,为45. 33% 和27. 51%;④优势科是乳杆菌科(35. 30%)和肠杆菌科(27. 51%);⑤乳杆菌属和埃希氏菌属为优势属(35. 30%?27. 49%);⑥在种的水平,可培养细菌的丰度为37. 10%,未分类细菌27. 69%,不可培养细菌35. 21%?可培养细菌中,唾液链球菌的丰度最高(26. 66%)?在丰度最高的20 个种中,8 种细菌(40%)未分类,为以往未被发现的新种?个体间细菌群落存在一定差异?结论 树鼩大肠内容物中的细菌组成具有丰富的多样性,其中还有许多未被分类鉴定且相对丰度较高的细菌,需要进一步研究?
英文摘要:
      Objective To analyze the structure and composition of the tree shrew hindgut microbiota by Illumina PE250 sequencing technology. Methods Three male 10?month?old tree shrews were selected. The V3 - V4 region was amplified with universal primers of bacteria 16SrDNA and sequenced with the Illumina PE250 platform after the extraction of total DNA. Results A total of 160 157 valid sequences and 437 operational taxonomic units (OTUs) were obtained. The bacteria in the tree shrew hindgut are from 9 phyla, 19 classes, 37 orders, 68 families, 137 genera, and 194 species. Among them, (i) Firmicutes and Proteobacteria had the highest abundance, at 63. 30% and 28. 52%, respectively. (ii) The dominant classes were Bacilli and Gammaproteobacteria ( 51. 46%, 28. 02%). ( iii ) The abundances of Lactobacillales and Enterobacteriales were high, at 45. 33% and 27. 51%. (iv) Dominant families were Lactobacillaceae (35. 30%) and Enterobacteriaceae (27. 51%). (v) Lactobacillus and Escherichia?Shigella were the dominant genera (35. 30%, 27. 49%). (vi) At the species level, the abundance of cultured bacteria was 37. 10%, that of unclassified bacteria was 27. 69%, and that of uncultured bacteria was 35. 21%. Lactobacillus salivarius was the most abundant species (26. 66%) among the cultured bacteria. Of the 20 species with the highest abundance, eight (40%) were unclassified and new species. Some differences among individuals in terms of bacterial communities were identified. Conclusions The tree shrew hindgut microbiota has rich diversity, and there are many bacteria that have not been identified and have relatively high abundance, which needs further study.
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