六年实验动物病原菌检测能力验证结果分析
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(中国食品药品检定研究院,实验动物资源研究所,北京 100050)

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xjvet@ nifdc. org. cn

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R-33

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Analysis of proficiency testing results for pathogenic bacteria in laboratory animal in six years
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(National Institutes for Food and Drug Control, Department of Laboratory Animal Quality Testing, Beijing 100050, China)

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    摘要:

    目的 总结分析2011 年?2013 ~2017 年实验动物病原菌检测能力验证活动情况,提高检测能力,促进能力验证活动开展?方法 2011 年?2013 年~2017 年,本院组织开展了六次7 个项目的实验动物病原菌检测能力验证活动?通过汇总六年中能力验证活动的结果数据,分析整体趋势,总结归纳出现的问题?结果 六年中共有全国20 个省市的45 个单位先后报名参加实验动物病原菌的能力验证活动,项目包括肺支原体?泰泽病原体?绿脓杆菌?金黄色葡萄球菌?沙门菌?肺炎克雷伯杆菌和支气管鲍特杆菌;满意率分别为75%?87.5%?80.0%?78.6%?93.3%?96.2%和88.0%?不满意结果原因主要有培养时间不足?可疑菌落筛选错误?生化鉴定错误?报告书写笔误以及未及时提交报告等?结论 通过连续的能力验证活动,国内实验动物病原菌检测水平逐渐提高,达到预期目标?

    Abstract:

    Objective To analyze the result of proficiency testing (PT) of detection activities for Laboratory animal pathogenic bacteria in 2011 and 2013 -2017. To further improve the detection capacity of laboratory animal testing agency, and promote PT to be carried out in future. Methods During the six years (2011 and 2013 - 2017), the National Institutes for Food and Drug Control conducted a total of six (seven projects) PT activities of laboratory animal pathogen bacteria. We analyzed the overall trend and the exposed problems by summarizing the result data of the PT in 6 years. Results A total of 45 laboratories in the country including 20 provinces and cities participated in the PT. The PT projects included Mycoplasma pulmonis, Clostridium piliformis, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella spp. , Klebsiella pneumoniae and Bordetella bronchiseptica. The satisfaction rates were 75%, 87.5%, 80.0%,78.6%, 93.3, 96.2% and 88.0%, respectively. The main reasons of unsatisfactory results were for lack of incubation time, select errors of suspicious bacteria, biochemical identification errors, report writing errors and not timely feedback results. Conclusions The level of domestic laboratory animal pathogenic bacteria detection is gradually increased to achieve the desired goal through continuous proficiency testing activities.

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邢进,冯育芳,王洪,张雪清,付瑞,岳秉飞.六年实验动物病原菌检测能力验证结果分析[J].中国比较医学杂志,2018,28(4):103~107.

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  • 收稿日期:2017-10-24
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  • 在线发布日期: 2018-05-22
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