系统性红斑狼疮MRL/ lpr 小鼠的免疫作用机制
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(广东省医学实验动物中心,广东佛山 528248)

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kuangss@126. com

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R-33

基金项目:

广东省科技计划基金(2013B060300033);广东省科技计划项目(2016A030303026)


Exploration of the immune mechanism in systemic lupus erythematosus MRL / lpr mice
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(Guangdong Medical Laboratory Animal Center,Foshan 528248,China)

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    摘要:

    目的 探讨不同月龄系统性红斑狼疮MRL/ lpr 小鼠免疫作用机制,为其发病机制的研究提供依据?方法 本研究随机选取SPF 级3 月龄?4 月龄?5 月龄及6 月龄MRL/ lpr 雌性小鼠各10 只,以及SPF 级3 月龄野生型C57 雌性小鼠10 只,测定脏器质量计算脏器系数?运用ELISA 方法检测血清抗双链DNA 抗体(ds-DNA)?脾组织白介素IL-2?IL-4?IL-17 和肿瘤坏死因子TNF-α,运用流式细胞技术检测脾淋巴细胞CD3 细胞含量和CD4/ CD8 细胞比率?结果 与3 月龄野生型C57 小鼠相比,3 ~ 6 月龄MRL/ lpr 小鼠脾脏系数?血液中ds-DNA 抗体浓度?IL-2 和TNF-α 浓度均显著升高( P < 0.05);白介素IL-4 浓度差异无显著性( P > 0.05);与3 月龄MRL/ lpr 小鼠比较, 5?6 月龄的MRL/ lpr 小鼠血液中IL-17 浓度显著降低( P < 0.05 或 P < 0.01);其余各项指标MRL/ lpr 小鼠不同月龄间差异无显著性?与3 月龄C57 小鼠相比, 6 月龄MRL/ lpr 小鼠脾淋巴细胞CD3 浓度显著降低( P < 0.01),随着月龄增大,MRL/ lpr 小鼠的CD3 和D4/ CD8 比率都有下降趋势,但差异无显著性?结论 在本实验条件下,各指标变化均显示3 月龄红斑狼疮MRL/ lpr 小鼠出现免疫损伤,并且损伤程度随小鼠月龄的增长呈一定的递增趋势?

    Abstract:

    Objective To explore the immune mechanism in the systemic lupus erythematosus MRL/ lpr mice at different months of age, and to provide the basis for research of its pathogenesis. Methods 3-, 4-, 5- and 6-month old female MRL/ lpr mice, and wild type C57 female mice were used in this study, 10 mice per each group. Their organ coefficients were determined. ELISA was performed to detect the serum levels of double stranded DNA ( ds-DNA) antibody. The interleukins IL-2, IL-4, IL-17 and tumor necrosis factor-α (TNF-α) in spleen tissue were detected. Flow cytometry was used to assess the content of spleen lymphocyte CD3 cells and CD4/ CD8 cell ratio. Results Compared with the 3-month old wild-type C57 mice, the spleen coefficient, the blood concentration of ds-DNA antibody, IL-2 and TNF-α in the 3- to 6-month old MRL/ lpr mice were significantly increased ( P < 0.05). There was no significant difference between the concentrations of interleukin IL-4 ( P > 0.05). The blood concentration of IL-17 in the 5- and 6-month old MRL/ lpr mice was significantly lower ( P < 0.05 or P < 0.01) than that in the 3-month old MRL/ lpr mice. The rest indexes of MRL/ lpr mice showed no obvious changes or significant difference in the mice at different ages. Compared with the 3-month old C57 mice, the spleen CD3 lymphocyte concentration in the MRL/ lpr mice was significantly decreased ( P < 0.01). With the increasing age, the CD3 lymphocyte concentration and D4 + / CD8 + cell ratio in the MRL/ lpr mice were decreased, however, showing a non-significant difference ( P ﹥ 0.05). Conclusions The data obtained in this study indicate that 3-month old lupus MRL/ lpr mice have already immune injury, increasing with the increase of age of the mice.

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邝少松,杨林,严家荣,谭巧燕,代路路,唐小江.系统性红斑狼疮MRL/ lpr 小鼠的免疫作用机制[J].中国比较医学杂志,2018,28(4):38~42.

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  • 收稿日期:2017-09-14
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  • 在线发布日期: 2018-05-22
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