• 首页
  • 期刊介绍
  • 编委会
  • 投稿指南
  • 期刊订阅
  • 广告合作
  • 留言板
  • 联系我们
  • English
楼琦,石巧娟,郭红刚,李巍,卢领群,周文伟,萨晓婴.非酒精性脂肪肝大鼠脂质代谢及病理变化的动态观察[J].中国比较医学杂志,2012,(3):5~11.
非酒精性脂肪肝大鼠脂质代谢及病理变化的动态观察
The Dynamic Observation of Lipid Metabolism and Pathological Changes in Non-alcoholic Fatty Liver Rats
  
DOI:10.3969/j.issn.1671.7856.2012.03.002
中文关键词:  非酒精性脂肪肝  胰岛素抵抗  细胞凋亡  病理  脂质代谢
英文关键词:Nonalcoholic fatty liver disease(NAFLD)  Insulin resistance(IR)  Apoptosis  Pathology  Lipid metabolism
基金项目:“十一五”科技支撑计划重点项目(2009BAI83B02);浙江省医药卫生计划项目(2006QN001, 2009A001)。
作者单位E-mail
楼琦 浙江省医学科学院实验动物中心,杭州310013 louqi@126.com 
石巧娟 浙江省医学科学院实验动物中心,杭州310013
浙江大学医学院药理学教研室 杭州310031 
 
郭红刚 浙江省医学科学院实验动物中心,杭州310013  
李巍 浙江省医学科学院实验动物中心,杭州310013  
卢领群 浙江省医学科学院实验动物中心,杭州310013  
周文伟 浙江省医学科学院实验动物中心,杭州310013  
萨晓婴 浙江省医学科学院实验动物中心,杭州310013 saxiaoyin@163.com 
摘要点击次数: 2718
全文下载次数: 1893
中文摘要:
      目的通过高脂饮食建立NAFLD大鼠模型,连续监测4~16周模型动物肝功能、脂质代谢、胰岛素抵抗及肝细胞凋亡在NAFLD进展过程中的变化情况及相互关系,为该模型在脂肪肝发病机制、脂肪肝治疗药物评价等方面的应用提供参考依据。方法SD大鼠50只,除正常对照组外,其余动物饲喂高脂饲料,分别检测4,8,12,16周大鼠血清GLU、CHO、TG、HDL、LDL、GPT、GOT及胰岛素水平,肝脏组织切片进行病理学及细胞凋亡观察,进一步分析大鼠肝功能、脂质代谢、胰岛素抵抗及肝细胞凋亡对肝组织病理改变的影响。结果模型组大鼠4周后就出现肝功能损伤,脂质代谢紊乱、胰岛素抵抗,肝细胞凋亡8 W后明显增加,肝细胞脂变及炎症为肝组织病理变化的主要特征,且造模时间越长,病变程度越严重。结论经过高脂饲料的喂养,SD大鼠在4~16周内可形成病变程度逐步加重的NAFLD模型,肝功能损伤,脂质代谢紊乱及肝细胞凋亡是引起非酒精性脂肪肝中脂肪变性和炎症的重要因素,该模型可应用于脂肪肝治疗药物评价等方面。
英文摘要:
      ObjectiveTo investigate the pathogenesis of nonalcoholic fatty liver disease(NAFLD), provide a reference for the evaluation of fatty liver therapeutic effect, the liver function, lipid metabolism,insulin resistance and liver apoptosis of NAFLD rats established by high cholesterol feeding were continuously monitored from 4~16 weeks. Methods 40 NAFLD rats established by high cholesterol feeding were randomly divided into the 4,8,2,6 weeks model group and a group of normal rats were set up for control, the serum GLU、CHO、TG、HDL、LDL、GPT、GOT and insulin were tested, the liver apoptosis and pathological changes were observed, then analyzed the effect of the liver function, lipid metabolism,insulin resistance and apoptosis on the pathological changes in non-alcoholic fatty liver rats. ResultsAfter 4 weeks of high cholesterol feeding, the rats had got the liver injury, disorder of lipid metabolism, insulin resistance. Liver cell apoptosis were increased obviously, and pathological changes were characterized by cell steatosis and inflammatory, the lesions became more serious with time went on. ConclusionsRats established by high cholesterol feeding could get the nonalcoholic fatty liver disease and the lesions became more serious with time over during 4~16 weeks. Lipid metabolism disorder and insulin resistance were the important cause of liver cell steatosis and inflammatory. The model we established is suitable for the evaluation of fatty liver therapeutic effect.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1653405 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会 中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码