To obtain the full-length coding sequence of the tree shrew lecithin-cholesterol acyltransferase（tsLCAT） gene and perform its molecular characterization. Methods Total RNA was extracted from tree shrew liver tissue, and RACE（Rapid Amplification of cDNA Ends） technology was used to clone the full-length cDNA of tsLCAT. MEGA software was employed to construct a phylogenetic tree for evolutionary relationship analysis. Tools such as ExPASy-ProtParam, SignaIP, and MusiteDeep were used to predict molecular characteristics and biological functions, while AlphaFold3 was utilized for protein structure prediction and alignment. RT-qPCR and Western Blot were performed to detect the mRNA and protein expression levels of tsLCAT in 11 tissues including liver, pancreas, and kidney. Results Two transcript variants of tsLCAT were obtained: tsLCAT X1（full length 1384 bp, encoding 440 amino acids） and tsLCAT X2（full length 1400 bp, encoding 438 amino acids）, with tsLCAT X1 being the major transcript. Phylogenetic analysis revealed that tsLCAT has a closer evolutionary relationship with primates than with rodents（such as mice and rats）, and tsLCAT showed the highest amino acid sequence identity with human and chimpanzee LCAT. Tissue expression profile indicated that tsLCAT mRNA had the highest expression level in the liver, and the protein was enriched in the liver and pancreas. Conclusion The full-length coding sequence of tsLCAT was successfully obtained and characterized, with systematic analysis of its molecular characteristics, protein structure, and biological functions, providing key molecular evidence for the use of tree shrews as a research model for LCAT-related diseases.