心血管类器官模型的研究进展
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1.浙江中医药大学;2.浙江省中医院

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国家自然科学基金面上项目(No.81973579),浙江省公益技术应用研究(实验动物)项目(No.2017C37182),浙江省中医药科研(No.2023ZL411、No.2023ZL422)


Research progress of cardiovascular organoid models
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1.Zhejiang University of Traditional Chinese Medicine;2.Zhejiang Provincial Hospital of Traditional Chinese Medicine

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    摘要:

    心血管疾病是当前全球发病率和死亡率最高的疾病,但其发病机制研究和药物开发仍面临重大挑战,部分归因于传统研究模型的局限性。二维(two dimensional,2D)细胞培养难以模拟体内微环境,而动物模型则存在物种差异,无法完全重现人类心血管系统的生理和病理特征。近年来,伴随干细胞技术不断突破,类器官模型研究迅速发展,为心血管机制研究和药物研发提供了创新工具。心血管类器官是由干细胞在体外三维(three dimensional,3D)培养条件下构建的微组织模型,可高度模拟人体心脏和血管的结构、组成及功能,显著提升研究的生理相关性。本文系统综述了其构建策略、应用进展,并探讨了当前局限与未来方向。

    Abstract:

    Cardiovascular disease is currently the disease with the highest incidence and mortality rate globally, but its pathogenesis research and drug development still face significant challenges, partly due to the limitations of traditional research models. Two dimensional (2D) cell cultures are difficult to simulate the microenvironment in vivo, while animal models have species differences that cannot fully reproduce the physiological and pathological characteristics of the human cardiovascular system. In recent years, with the continuous breakthrough of stem cell technology, organoid model research has developed rapidly, providing innovative tools for cardiovascular mechanism research and drug development. Cardiovascular organoids are microtissue models constructed by stem cells under three dimensional (3D) culture conditions in vitro, which can highly simulate the structure, composition and function of the human heart and blood vessels, significantly improving the physiological relevance of research. This paper systematically reviews its construction strategies and application progress, and discusses its current limitations and future directions.

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历史
  • 收稿日期:2025-08-08
  • 最后修改日期:2025-12-10
  • 录用日期:2026-03-09
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