不同穴位艾灸对薄型子宫内膜的修复作用研究
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广州中医药大学

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Study on repairing effect of moxibustion at different acupoints
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1.Guangzhou University of Chinese Medicine;2.Zhongshan Hospital of Traditional Chinese Medicine

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    摘要:

    目的:比较艾灸“关元“和”肾俞“穴对薄型子宫内膜大鼠的修复作用。方法:将SD大鼠随机分为四组,使用95%无水乙醇建立薄型子宫内膜模型。关元组和肾俞组分别艾灸“关元”和”肾俞“穴,其余两组行相同固定。测量艾灸前后不同时间穴区温度变化,HE染色法观察子宫病理形态,ELISA法检测血清炎症因子及激素水平,免疫组化法检测子宫增殖和容受性因子表达,Western blot法检测血管生成因子的表达。结果:艾灸后即刻和艾灸后5min肾俞穴区温度均高于关元 (P<0.001,P<0.01)。造模后大鼠子宫内膜厚度、腺体及血管数量均减少;血清IL-1β升高,PROG降低;子宫Ki67、PCNA、LIF、ITG-β3,ANG-1、CD34表达均降低。艾灸关元和肾俞均能增加内膜厚度、腺体和血管数,降低IL-1β、增加E2和PROG水平,肾俞也升高IL-10水平(P<0.05);两组 Ki67、PCNA、LIF、ITG-β3表达增强,关元组PCNA表达高于肾俞组(P<0.05),肾俞组LIF、ITG-β3表达强于关元组(P<0.01,P<0.05);两组CD34、VEGFA表达升高(P<0.0001),关元组ANG-1表达高于肾俞组(P<0.0001),而肾俞组CD34表达更高(P<0.05)。结论:艾灸“关元”穴和”肾俞”穴均能修复薄型子宫内膜,关元穴在降低IL-1β、提高E2水平、促进增殖因子的表达上优于肾俞穴,肾俞穴在提高PROG水平、促进子宫内膜容受性因子的表达上优于关元穴,两穴在促进血管生成方面可能通过不同的途径发挥作用。

    Abstract:

    Objective: To compare the repair effects of moxibustion "Guanyuan" and "Shenshu" on thin endometrium rats. Methods: 32 SD rats were randomly divided into normal group, model group, Guan Yuan group and Shenshu group with 8 rats in each group. Thin endometrium model was established using 95% anhydrous alcohol. After two estrus cycles, the Guanyuan group and Shenshu group moxibusted "Guanyuan" and "Shenshu" points, once a day, and rested for 1 day every 6 days for a total of 21 days. The other two groups were fixed in the same way. The temperature changes in the acupoint area at different time were measured by infrared thermometer, the pathological morphology of uterus was observed by HE staining, and serum levels of IL-1β, IL-10, E2 and PROG were detected by ELISA. The expressions of Ki67, PCNA, LIF and ITG-β3 in uterus were detected by immunohistochemistry. The expression levels of ANG-1、CD34和VEGFA in uterus were detected by Western blot. Results: Immediately after moxibustion and 5min after moxibustion, the body surface temperature at Shenshu point was higher than that at Guanyuan point (P<0.001, P<0.01). Compared with normal group, endometrial thickness, number of glands and blood vessels in model group were decreased (P<0.0001, P< 0.01, P<0.001), The endometrial thickness, number of glands and blood vessels increased in Guanyuan group (P<0.0001, P<0.01, P<0.01) and Shenshu group (P < 0.0001, P<0.01, P<0.05),and the endometrial thickness, number of glands and blood vessels in Guanyuan group were better than those in Shenshu group, but the difference was not statistically significant. Compared with normal group, the serum IL-1β level in model group was increased (P<0.01), and the PROG level was decreased (P<0.001). Compared with model group, the serum IL-1β in Guanyuan group were decreased (P<0.01), E2 level and PROG level increased (P<0.01, P<0.05),and the serum IL-1β were decreased (P<0.05), IL-10,E2 and PROG levels (P<0.05, P<0.05, P<0.01) were increased in Shenshu group. Compared with normal group, the expressions of Ki67, PCNA, LIF and ITG-β3 of model group were decreased (P< 0.0001). Compared with model group, the expressions of Ki67, PCNA, LIF and ITG-β3 in Guanyuan group (P<0.0001) and Shenshu group (P<0.001, P<0.001, P<0.0001, P<0.001) were increased, and PCNA expression in Guanyuan group was higher than that in Shenshu group (P<0.05). The expressions of LIF and ITG-β3 in Shenshu group were stronger than those in Guanyuan group (P<0.01, P<0.05). Compared with normal group, the expression of ANG-1 and CD34 protein in uterine tissue of model group was decreased (P<0.0001). Compared with model group, the expressions of ANG-1, CD34 and VEGFA in Guanyuan group were increased (P<0.0001), and the expressions of CD34 and VEGFA in Shenyu group were increased (P<0.0001). The expression of ANG-1 in Guanyuan group was higher than that in Shenyu group (P<0.0001), while the expression of CD34 in Shenyu group was higher than that in Guanyuan group (P<0.05). Conclusion: Moxibustion "Guanyuan" point and "Shenshu" point could repair thin endometrium, Guanyuan point is superior to Shenshu point in reducing the level of inflammatory factors, increasing the level of E2, promoting the expression of proliferation factors, while Shenshu point is superior to Guanyuan point in increasing the level of anti-inflammatory factors and PROG, and promoting the expression of endometrium receptor related factors. The two points may play a role in promoting angiogenesis through different ways.

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  • 收稿日期:2024-06-18
  • 最后修改日期:2024-08-12
  • 录用日期:2024-10-30
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