人参皂苷Rg1、Rb1 通过调控炎症改善慢性不可预测应激致大鼠抑郁、焦虑样行为的作用及比较
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1.湖南中医药大学;2.中国医学科学院药用植物研究所

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中国医学科学院创新工程“人类疾病动物模型平台(神经精神疾病模型行为实验分析分题)”(2021-1-I2M-034);


Effects and comparison of ginsenosides Rg1 and Rb1 depression and anxieti-like behavior induced by chronic unpredictable stress in rats by regulating inflammation
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1.Hunan University of Chinese Medicine;2.Ningbo University

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    摘要:

    目的:研究人参皂苷Rg1、Rb1 通过调控炎症改善慢性不可预测应激诱导大鼠的抑郁样和焦虑样行为的作用及比较。 方法:SPF级SD雄性大鼠70只,适应 5 天后进行糖水偏爱实验检测,根据糖水偏爱指数将动物分为7组,即空白对照组、模型组、阳性药组、人参皂苷 Rg1 30 μmol/kg 剂量组、人参皂苷 Rg1 60 μmol/kg剂量组、人参皂苷 Rb1 30 μmol/kg剂量组、人参皂苷 Rb1 60 μmol/kg剂量组。除空白对照组外,其余大鼠每日随机接受1-2种不同的刺激,造模时间共35d。于第36天进行糖水偏爱、旷场实验、新奇环境摄食抑制实验、大鼠高架十字迷宫实验、强迫游泳等行为学实验,考察其抗抑郁、抗焦虑作用。采用Elisa法测定大鼠血清和海马IL-1β、IL-6、TNF-α炎症因子水平,和血清皮质酮(CORT)水平。结果:与模型组相比,人参皂苷Rg1、Rb1组大鼠糖水偏爱指数提升,强迫游泳不动时间显著减少,人参皂苷 Rg1(60 μmol/kg)组新奇抑制摄食潜伏期显著减少,人参皂苷 Rg1(30 和 60 μmol/kg)组开臂时间的比例显著上升,人参皂苷Rg1、Rb1两个剂量组血清中皮质酮的含量显著减少,人参皂苷 Rg1(30 μmol/kg) 剂量组血清中 IL-1β 和 IL-6的水平显著降低,人参皂苷 Rb1 (30 μmol/kg) 剂量组血清中 TNF-α 和 IL-6的水平显著降低,人参皂苷 Rg1、 Rb1 高剂量组海马中 IL-1β、IL-6 和TNF-α 的含量显著降低。 结论:两种人参皂苷均可通过调节 HPA 轴、抑制神经炎症,从而改善慢性不可预测应激致大鼠抑郁、焦虑样行为,此外人参皂苷 Rg1 的抗焦虑作用显著优于Rb1。

    Abstract:

    Objective: To study the effect and comparison of ginsenosides Rg1 and Rb1 on depression-like and anxiety-like behavior in chronic unpredictable stress-induced rats by regulating inflammation. Methods: Seventy SPF grade SD male rats were tested for sugar and water preference after 5 days of adaptation. The animals were divided into 7 groups according to the sugar and water preference index, namely blank control group, model group, positive drug group, ginsenoside Rg1 30 μmol/kg dose group, ginsenoside Rg1 60 μmol/kg dose group, ginsenoside Rb1 30 μmol/kg dose group, ginsenoside Rb1 60 μmol/kg dose group. Except for the blank control group, the other rats were randomly subjected to 1-2 different stimulating factors every day for a total of 35 days. On the 36th day, behavioral experiments such as sugar and water preference, open field experiment, novel environment feeding inhibition experiment, elevated cross maze experiment and forced swimming were conducted to investigate its anti-depression and anti-anxiety effects. The serum and hippocampal levels of IL-1β, IL-6, TNF-α and serum corticosterone (CORT) were measured by Elisa. Result: Compared with model group, ginsenoside Rg1 and Rb1treatments significantly increased the sucrose consumption in the sucrose preference test and decrease in immobility in the forced swimming test., the latency to eat in novelty-suppressed feeding test of ginsenoside Rg1 (60 μmol/kg) group was significantly reduced, and percentage of open arm entries and time in elevated plus maze test of ginsenoside Rg1 (30 and 60 μmol/kg) groups was significantly increased. The content of serum corticosterone in ginsenoside Rg1 and Rb1 dose groups was significantly decreased. The levels of serum IL-1β and IL-6 in ginsenoside Rg1 (30 μmol/kg) dose group were significantly decreased, The levels of TNF-α and IL-6 in serum of ginsenoside Rb1 (30 μmol/kg) group were significantly decreased. And the contents of IL-1β, IL-6 and TNF-α in hippocampus of ginsenoside Rg1 and Rb high dose group were significantly decreased. Conclusion: Both ginsenosides can regulate the HPA axis and inhibit neuroinflammation, improving depression and anxieti-like behavior in rats induced by chronic unpredictable stress. In addition, ginsenoside Rg1 has a significantly better anti-anxiety effect than Rb1.

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  • 收稿日期:2023-12-13
  • 最后修改日期:2024-04-20
  • 录用日期:2024-04-22
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