脊髓灰质炎疫苗脑内法猴体神经毒力试验的神经病理研究
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中国医学科学院医学实验动物研究所

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中国医学科学院医学与健康科技创新工程(2022-I2M-1-012)


Neuro-pathological study of the intrathalamic neurovirulence test of poliomyelitis vaccine in rhesus macaques
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1.Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences Comparative Medicine Center,Peking Union Medical College;2.Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Beijing 100021;3.China

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CAMS Innovation Fund for Medical Sciences(2022-I2M-1-012)

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    摘要:

    目的 本研究为探讨脊髓灰质炎疫苗猴体神经毒力试验毒力返强对神经免疫反应的影响及病理机制。方法 I型、II型和III型Sabin株脊髓灰质炎灭活疫苗(Vero细胞)的原液(不低于7000 lgCCID50?L-1)以及10-1稀释各型脊髓灰质炎疫苗采用脑内法进行神经毒力试验,观察脊髓灰质炎的病理变化,并利用免疫组织化学法检测脊髓灰质炎病毒受体CD155以及CD4+T淋巴细胞、CD20+B淋巴细胞和CD68+小胶质细胞的分布。结果 发生脊髓灰质炎的猴体出现脊髓炎症细胞浸润,少量神经元变性,脊髓神经元变性坏死、卫星现象、噬神经细胞现象、血管周围炎症细胞袖套状浸润和胶质细胞增生;主要为CD4+T淋巴细胞、CD20+B淋巴细胞和CD68+小胶质细胞大量浸润在血管袖套和增生的胶质结节中;在发生和未发生脊髓灰质炎的猴体中,CD155分布未见明显差别,均表达在神经元和胶质细胞,而血管内皮细胞未见表达。结论 脊髓灰质炎疫苗毒力返强导致典型病毒性脑脊髓炎。

    Abstract:

    Objective To study the effect and pathological mechanisms of the neuro-immune response of viral encephalomyelitis caused by the virulence reversion of the intrathalamic neurovirulence test of poliomyelitis vaccine in rhesus macaques. Methods The stock solution (not less than 7000 lgCCID50?L-1) of the inactivated polio vaccine (Vero cells) of type I, type II and type III Sabin strains and 10-1 dilution of each type of polio vaccine were carried out using intrathalamic neurovirulence test. We observed the pathological changes of polio. Via immunohistochemical method, the distribution of receptor CD155 and CD4+ T lymphocytes, CD20+ B lymphocytes and CD68+ microglia were detected. Results Lesions were observed on the virulence reverted polio case. Inflammatory cells infiltrating, neuronal degeneration and necrosis, satellite phenomenon, perivascular cuffing and glial cell proliferation were observed in the spinal cord. The inflammatory cells were mainly CD4+ T lymphocytes, CD20+ B lymphocytes, and CD68+ microglia in perivascular cuffing and proliferative glial nodules. There is no significant difference on distribution of poliovirus receptor CD155 in neurons and glial cells of monkeys with and without polio, while no expression was observed in vascular endothelial cells. Conclusion Polio caused by virulence reversion of the intrathalamic neurovirulence test is viral encephalomyelitis.

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  • 收稿日期:2023-11-13
  • 最后修改日期:2024-03-18
  • 录用日期:2024-03-22
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