散结祛瘢方对BALB/C裸鼠瘢痕疙瘩模型的作用及转化生长因子-β1的影响
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1.北京中医药大学;2.中日友好医院

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1.国家自然科学基金青年项目(编号:82205115)2.中央高水平医院临床科研业务费资助(编号:ZRJY2023-QM12) 3.中日友好医院“菁英计划”人才培育工程资助(编号:ZRJY2023-QM12)


Effect of Sanjie Quban Recipe on the Keloid Model of BALB/C Nude Mice and Influence on Transforming Growth Factor-β1
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1.Beijing University of Chinese Medicine;2.China-Japan Friendship Hospital

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    摘要:

    目的:探讨散结祛瘢方对瘢痕疙瘩裸鼠模型的作用及转化生长因子-β1(transforming growth factor-β1, TGF-β1)的影响。方法:将人手术切除后瘢痕疙瘩组织移植至6~8周龄健康SPF级BALB/C 雌性裸鼠背部皮下,建立瘢痕疙瘩裸鼠模型,随机分成散结祛瘢方组、积雪苷片组、对照组3组,每组5只,分别用散结祛瘢方、积雪草苷片及无菌纯水进行干预,连续灌胃28天后剥取瘢痕疙瘩组织,进行称重、苏木精伊红(hematoxylin eosin, HE)染色、Masson染色、免疫组化(TGF-β1)染色。比较三组间治疗前后的瘢痕疙瘩重量差值的差异,以及比较三组间治疗后的胶原纤维、成纤维细胞数量及TGF-β1表达的差异。结果:积雪草苷片组的瘢痕疙瘩重量差值大于对照组,且散结祛瘢方组与积雪草苷片组、对照组相比,散结祛瘢方组瘢痕疙瘩治疗前后重量差值最大(P < 0.01);散结祛瘢方组较对照组胶原纤维疏松、数量减少;成纤维细胞数量减少;散结祛瘢方组较对照组TGF-β1表达减少(P < 0.01)。结论:散结祛瘢方对瘢痕疙瘩具有一定的治疗作用,其机制可能是通过减少瘢痕疙瘩组织中TGF-β1的表达,从而减少成纤维细胞的增殖与细胞外基质的合成。该研究为临床中用中药治疗瘢痕疙瘩提供了实验基础与理论依据。

    Abstract:

    Objective This study aimed to investigate the therapeutic efficacy of the Sanjie Quban Recipe on a keloid nude mice model and its impact on Transforming Growth Factor-β1 (TGF-β1). Methods Keloid tissue after surgical resection was transplanted into the subcutaneous back of healthy SPF BALB/C female nude mice aged 6-8 weeks, and the keloid nude mice model was established. The mice were randomly divided into 3 groups, 5 in each group. They were respectively treated with Sanjie Quban Recipe, Asiaticoside tablet and sterile pure water. After 28 days of continuous gavage, keloid tissue was exfoliated and weighed, HE staining, Masson staining, and immunohistochemical staining (TGF-β1). The difference of keloid weight between the three groups before and after treatment was compared, and the difference of collagen fiber, fibroblast number and TGF-β1 expression between the three groups after treatment was also compared. Results The weight difference of keloid in the Asiaticoside tablet group was greater than that in the control group, and the weight difference before and after keloid treatment was the largest in the Sanjie Quban Fang group compared with the Asiaticoside tablet group and the control group (P < 0.01). Compared with the control group, the collagen fibers in Sanjie Quban Fang group were loose and the number decreased. The number of fibroblasts decreased. The expression of TGF-β1 in Sanjie Quban Formula group was decreased compared with control group (P < 0.01). Conclusion Sanjie Quban Recipe has certain therapeutic effect on keloid, the mechanism may be through reducing the expression of TGF-β1 in keloid tissue, thereby reducing the proliferation of fibroblasts and the synthesis of extracellular matrix. This study provides experimental basis and theoretical basis for the clinical treatment of keloid with Chinese medicine.

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  • 收稿日期:2023-09-21
  • 最后修改日期:2023-10-17
  • 录用日期:2024-02-26
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