精索静脉曲张合并勃起功能障碍动物模型的构建和评价
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1.广西中医药大学研究生院;2.广西中医药大学附属瑞康医院;3.桂林市中医医院

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国家自然科学基金项目(82160905,82260933);广西中医药大学研究生教育创新计划项目(YCBXJ2023019)


Establishment and evaluation of animal model of Varicocele and Erectile dysfunction
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Affiliation:

1.Graduate School of Guangxi University of Chinese Medine;2.Ruikang Hospital Affiliated to Guangxi University of Chinese Medine;3.Guilin Municipal Hospital of Traditonal Chinese Medicin

Fund Project:

The National Natural Science Foundation of China

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    摘要:

    目的 通过手术诱导SD大鼠精索静脉曲张(VC),阿扑吗啡试验(APO试验)筛选测VC后勃起功能障碍(ED)的大鼠,探讨建立VC合并ED模型的方法。方法 将60只大鼠随机分为空白组、假手术组、模型组,每组20只;参照Turner法,部分结扎左侧肾静脉,诱导左侧VC;并进行3次APO试验,筛选诱导VC后出现ED的大鼠,观察并记录勃起次数、生殖器梳理次数、打哈欠次数;测量双侧精索静脉直径;对双侧睾丸和肾脏称重;HE染色观察阴茎和左睾的病理改变;统计模型组大鼠的成模率。结果 模型组20只大鼠中有15只成功进行VC合并ED造模,成模率75%;造模后,模型组左侧精索静脉直径显著增加,且显著高于造模前,模型组右侧精索静脉直径增加,且高于造模前;模型组左侧睾丸重量显著降低,与右侧睾丸比较重量显著降低;各组双侧肾脏重量的比较,组间及组内无明显差异;模型组勃起次数、打哈欠次数、生殖器梳理次数显著减少,且随造模时间递减;模型组左侧睾丸和阴茎病理改变显著。结论 Turner法可以诱导大鼠精索静脉直径增加,引起睾丸损伤和重量下降,APO试验可以筛选诱导VC后出现ED的大鼠,二者相结合适用于构建与人类VC合并ED状态较相似的动物模型。

    Abstract:

    Objective By surgery inducing Varicocele(VC) in SD rats and Apomorphine Test(APO Test),we screened the rats with Erectile dysfunction(ED) after VC,and explored the method of establishing VC and ED model.Methods Randomly divide 60 rats into control group,sham group,and model group,with 20 rats in each group.Referring to the Turner method,partially ligate the left renal vein to induce left VC.And conduct three APO Tests to screen rats with ED after inducing VC,observe and record the number of erection,genital grooming,and yawning.Measuring the diameter of spermatic vein.Weighing both testises and kidneys.HE staining and observing the pathological changes in the penis and left testis.The success rate of modeling was calculated in the model group.Results 15 out of 20 rats in the model group were successfully established VC and ED model,with a success rate of 75%.After modeling,the diameter of the left spermatic vein in the model group increased significantly and were significantly higher than before-modeling,and the diameter of the right spermatic vein in the model group increased and were higher than before-modeling.The weight of the left testises in the model group significantly decreased compared to the right testises.There was no significant difference between or within groups in the comparison of bilateral kidney weight. In the model group,the number of erections,yawning and genital grooming decreased significantly,and decreased with the time of modeling.The pathological changes of the left testis and penis were significant in the model group.Conclusions Turner method can induce the increase of the diameter of spermatic vein in rats,causing testis injury and weight loss,and APO Test can screen the rats with ED after VC induction.The combination of the two methods is suitable for establishing an animal model with VC and ED status similar to human.

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  • 收稿日期:2023-08-08
  • 最后修改日期:2023-11-19
  • 录用日期:2024-02-26
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