江香薷炮制前后多糖含量及消炎止血抗氧化活性的影响
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江西中医药大学

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江西中医药大学校级科技创新团队发展计划(项目编号CXTD-22004?);江西中医药大学省级创新创业训练计划(项目编号: S202310412055)


Effects of polysaccharide content and anti-inflammatory, hemostatic and antioxidant activity before and after preparation of Mosla chinensis-jiangxiangru
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Jiangxi University of Traditional Chinese Medicine

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Jiangxi University of Chinese Medicine University-level Science and Technology Innovation Team Development Program (Project No. CXTD-22004); Provincial Innovation and Entrepreneurship Training Program of Jiangxi University of Chinese Medicine (Project No.: S202310412055)

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    摘要:

    摘要:目的:探究生姜汁炮制前后江香薷多糖含量变化,以及对炮制前后多糖消炎止血抗氧化活性评价。方法:利用苯酚-硫酸法测定炮制前后江香薷多糖(江香薷多糖JXRPs,姜制江香薷多糖JZJXRPs)的含量;体内通过构建大鼠足肿胀模型和体外脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7炎症模型,采用细胞增殖(MTT)法选取最佳给药浓度,ELISA法测定细胞上清液中IL-6、IL-12、NO、IL-4和IL-10的表达来评价炮制前后多糖的抗炎作用;通过剪尾法观察小鼠的出血时间来评价其止血效果;最后,利用清除DPPH和ABTS的能力来评价其体外抗氧化活性能力。结果:生姜汁炮制前后江香薷多糖的含量分别为13%,22%;大鼠足肿胀结果显示:在注射角叉菜胶4h后,与模型组相比,江香薷多糖在给药剂量为200 mg/kg出现显著性差异(P<0.05),而姜制江香薷多糖在给药剂量为100 mg/kg时就可明显减轻大鼠足肿胀(P<0.05);体外抗炎结果显示,炮制前后的多糖均能显著抑制细胞分泌IL-6、IL-12和NO(P<0.01),促进细胞分泌IL-4和IL-10(P<0.01),且炮制后作用更强。小鼠断尾止血实验结果表明:与对照组相比,江香薷多糖组可以加快止血效果但无显著性差异,而姜制江香薷多糖在中剂量时有显著性差异(P<0.05),而高剂量有加快趋势但并无显著性差异;体外抗氧化活性结果显示:炮制前后的江香薷多糖对DPPH和ABTS皆有不同程度的清除能力,其中江香薷多糖的IC50值分别为0.2215 mg/ml和0.2110 mg/ml,姜制江香薷多糖的IC50值分别为0.1651 mg/ml和0.1884 mg/ml。结论:江香薷经生姜汁炮制后可促进多糖的溶出,增加多糖含量;炮制后江香薷多糖消炎止血及抗氧化能力均比江香薷多糖较强,可为江香薷多糖后续研究及临床应用奠定基础。

    Abstract:

    Abstract:Objective:Exploring the changes in polysaccharides in Jiangxiangyu before and after giner juice, and evaluation of polysaccharide anti -inflammatory and antioxidant antioxidant activity before and after the processing. Methods: The content of polysaccharides beforeand after processing was determined by phenol-sulfuric acid method(Elsholtzia chinensis polysaccharide-JXRPs, Ginger Elsholtzia chinensis polysaccharide-JZJXRPs); The body internal uses the swelling model of rats and the body fat polysaccharide (LPS) to induce mousemacrophages RAW264.7 inflammation model.The best administration concentration is selected by using cell proliferation (MTT) method, Elisa method measures the expression of IL-6, IL-12, NO, IL-4, and IL-10 in the cells;Observe the bleeding time of mice to evaluate its hemostatic effect by observing the tail -cutting method; finally, use the ability to remove DPPH and ABTS to evaluate its in vitro antioxidant activity.Results: The contents of JXRPs and JZJXRPs were 13% and 22%,As a result of the swelling of rats, after 4h in injection, compared with the model group, JXRPs ppear significant difference in dosage doses of 200 mg/kg (P <0.05), and JZJXRPs When the dose is 100 mg/kg, you can significantly reduce the swelling of rats (P <0.05);The in vitro anti-inflammatory results show that the polysaccharides before and after the processing can significantly inhibit cell secretion of IL-6, IL-12 and NO (P <0.01), promote cell secretion of IL-4 and IL-10 (P <0.01), and the processing of the processing The post -effects are stronger.The results of the mouse break the hemostatic experiment show that: compared with the control group, the JXRPs group can speed up the hemostatic effect but there is no significant difference, and there is asignificant difference in the dosage of the JZJXRPs (P <0.05) in the medium dose (P <0.05), and the High doses have accelerated trend but there is no significant difference; The results of antioxidant activity in vitro showed that JXRPs and JZJXRPs had different degrees of scavenging ability for DPPH and ABTS, with IC50 values of JXRPs of 0.2215 mg/ml and 0.2110 mg/ml of JZXRPs, respectively, and IC50 values of 0.1651 mg/ml and 0.1884 mg/ml of JZJXRPs, respectively.Conclusion:After the Jiangxiangyu is produced by ginger juice, it can promote the dissolution of polysaccharides and increase the polysaccharide content. JZJXRPS anti -inflammatory hemostasis and antioxidant capacity are stronger than JXRPS, which can lay the foundation for the follow -up research and clinical application of JXRPS.

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  • 收稿日期:2023-07-15
  • 最后修改日期:2023-09-26
  • 录用日期:2024-01-16
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