miR-379-5p抑制小鼠乳腺癌4T1细胞的增殖、侵袭和迁移
DOI:
作者:
作者单位:

潍坊医学院

作者简介:

通讯作者:

中图分类号:

基金项目:

山东省自然科学基金项目(ZR2022MH311);山东省高等学校青创人才引育计划团队项目(205)


MiR-379-5p inhibits proliferation, invasion and migration of mouse breast cancer 4T1 cells
Author:
Affiliation:

Weifang Medical University

Fund Project:

Shandong Natural Science Foundation Project(ZR2022MH311);Introduction Plan of Young Creative Talents in Colleges and Universities of Shandong Province ( 205)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:通过研究miR-379-5p对小鼠乳腺癌4T1细胞增殖、侵袭和迁移的影响,为临床抑制乳腺癌增殖、侵袭和转移提供新的治疗靶点。方法:实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction, qRT-PCR)用来检测miR-379-5p在质粒转染后4T1细胞中的表达情况;采用(5-ethynyl-2’-deoxyuridine, EdU)细胞增殖实验与Transwell实验检测各组4T1细胞增殖能力与侵袭能力;通过划痕愈合实验检测过表达和敲低miR-379-5p后4T1细胞的迁移能力;利用BABL/c小鼠建立乳腺癌小鼠移植瘤模型,观察miR-379-5p过表达后对体内肿瘤生长状况,肺转移灶数量及大小的影响 。结果:EdU结果显示,与对照组相比,敲低miR-379-5p能增强细胞的增殖能力,过表达miR-379-5p能够抑制乳腺癌细胞的增殖能力(p<0.05);Transwell与伤口愈合实验结果显示,敲低miR-379-5p能够增强乳腺癌细胞的侵袭和迁移能力,过表达miR-379-5p显著抑制细胞的侵袭和迁移能力(p<0.01)。BABL/c小鼠体内成瘤实验显示,过表达miR-379-5p可显著减缓肿瘤的生长速度(p<0.05),对肺转移有抑制作用(p<0.01) 。结论:miR-379-5p在乳腺癌中起到抑癌基因的作用,抑制小鼠乳腺癌4T1细胞的增殖,侵袭和迁移。

    Abstract:

    By investigating the effects of miR-379-5p on the proliferation, invasion and metastasis of mouse breast cancer 4T1 cells, we provide new therapeutic targets for clinical inhibition of breast cancer proliferation, invasion and metastasis. Methods After plasmid transfection, 4T1 cells were utilized to detect the expression of miR-379-5p using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR); 5-ethynyl-2' doxyuridine (5-ethynyl-2'-deoxyuridine, EdU) cell proliferation assay and Transwell assay to detect changes in proliferation and invasion ability of 4T1 cells in each group; Migration ability of 4T1 cells after overexpression and knockdown of miR-379-5p was examined by scratch healing assay; BABL/c mice were used to establish the transplanted tumor model of breast cancer mice, and the tumor growth in vivo after miR-379-5p overexpression was observed.The transplanted tumor model of breast cancer was established in BABL/c mice, and the effects of overexpression of miR-379-5p on tumor growth and the number and size of lung metastases were observed . Results EdU results showed that knockdown of miR-379-5p enhanced the proliferation ability of cells compared with the control group, and miR-379-5p overexpression reduced the capacity of breast cancer cells to proliferate (p<0.05). Transwell and wound healing assays showed that knockdown of miR-379-5p enhanced the invasion and migration ability of breast cancer cells, and overexpression of miR-379-5p significantly inhibited the invasive and migratory ability of cells (p<0.01). In vivo tumorigenesis experiment of BABL/c mice showed that overexpression of miR-379-5p could significantly slow down the growth rate of tumor (p<0.05) and inhibit lung metastasis (p<0.01 ).In vivo experiments demonstrated that overexpression of miR-379-5p significantly slowed tumor growth (p<0.05). Conclusions miR-379-5p plays the role of tumor suppressor gene in breast cancer and inhibits the proliferation, invasion and migration of mouse breast cancer 4T1 cells.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-07-11
  • 最后修改日期:2023-10-09
  • 录用日期:2024-02-26
  • 在线发布日期:
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭