基于数据挖掘的急性肝损伤动物模型应用分析
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河南中医药大学

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岐黄学者;河南省重大公益专项


Analysis of application characteristics of acute liver injury animal model based on data mining
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Henan University of Traditional Chinese Medicine

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国中医药人教函2022-6;201300310100

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    摘要:

    目的 探究各类急性肝损伤动物模型的造模要素,为急性肝损伤动物模型的建立和评价提供参考和建议。方法 在中国知网(CNKI)、万方(WanFang)、重庆维普(VIP)、中华医学期刊全文数据(Yiigle)、PubMed等数据库检索2002-2022年急性肝损伤动物实验文献,归纳总结文献中涉及到的急性肝损伤动物模型的动物种类、阳性对照药、造模方法、造模药及给药剂量、给药时间、检测指标,应用Excel、SPSS Modeler18.0、Cytoscape3.8.2对结果进行分析。结果 数据库共纳入896篇文献,急性肝损伤动物模型中应用最多的动物是雄性KM小鼠;造模方法以化学性肝损伤、酒精性肝损伤、药物性肝损伤、免疫性肝损伤四大类为主,分别对应的主要造模方式为:① 实验前24h腹腔注射10.0 mL 0.1%CCl4植物油;② 实验前16h灌胃12.0 mL 50.0%-56.0%乙醇;③ 实验前24h腹腔注射APAP 300mg/kg;④ 实验前8h尾静脉注射Con A 20mg/kg。模型的评价以肝脏病理指标为金指标,结合生化指标血清ALT、AST,肝组织匀浆SOD、MDA含量及活性为直接指标。结论 临床上急性肝损伤发病原因各异,在制备急性肝损伤动物模型时应根据特定的研究内容、研究特点选择对应的造模方法。

    Abstract:

    Objective To investigate the modeling elements of various types of animal models for acute liver injury, and to provide references and suggestions for the establishment and evaluation of animal models for ALI. Methods The animal experimental literature of acute liver injury from 2002 to 2022 was searched in the databases of China Knowledge Network, WanFang, Chongqing Vip, Chinese Medical Journal Full Text Data, PubMed, etc. The animal species, positive control drugs, modeling methods, modeling drugs and drug administration of the animal models of acute liver injury involved in the literature were summarized and summarized. The results were analyzed using Excel, SPSS Modeler18.0 and Cytoscape3.8.2. Results A total of 896 papers were included in the database. The most used animal models for acute liver injury were male KM mice; the modeling methods were mainly chemical liver injury, alcoholic liver injury, drug-related liver injury and immune liver injury, and the corresponding main modeling methods were : ① intraperitoneal injection of 10.0 mL 0.1% CCl4 vegetable oil, 24 h before the experiment; ② gavage of 12.0 mL 50.0%-56.0% ethanol, 16 h before the experiment; ③intraperitoneal injection of APAP 300 mg/kg, 24 h before the experiment; ④ tail vein injection of Con A 20 mg/kg, 8 h before the experiment. The evaluation of the model was based on liver pathological indexes as the gold index, combined with biochemical indexes Serum ALT, AST, liver tissue homogenate SOD, MDA content and activity were used as direct indicators. Conclusions Since the causes of acute liver injury vary in clinical practice, the preparation of animal models of acute liver injury should be based on the specific study content and characteristics of the study, and the corresponding modeling methods should be selected.

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  • 收稿日期:2023-04-21
  • 最后修改日期:2023-05-25
  • 录用日期:2024-01-17
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