不同月龄C57BL/6小鼠大脑皮质功能区的微血管形态计量学分析
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山西医科大学实验动物中心

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Microvascular morphometric analysis of cerebral cortical functional areas in C57BL/6 mice of different months of age
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Laboratory Animal Center of Shanxi Medical University, Taiyuan

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    摘要:

    【摘要】目的 观察不同月龄C57BL/6小鼠大脑皮质不同功能区的微血管构筑,并进行定量分析。方法 采用改良碱性磷酸酶染色法染色C57BL/6小鼠大脑皮质运动皮质区(初级运动皮层、次级运动皮层)、感觉皮质区(初级体感皮层、次级体感皮层)、视觉皮质区(初级视觉皮层、次级视觉皮层)、听觉皮质区(初级听觉皮层、次级听觉皮层)、嗅觉皮质区(外嗅皮层、内嗅皮层)的微血管形态,OLMPUS BX51显微镜结合Image-Pro Plus 5.1进行图像捕获,采用Image-Pro Plus 5.1软件进行微血管长度密度(Lv)、微血管表面积密度(Sv)、微血管体积密度(Vv)的定量分析。结果 C57BL/6小鼠大脑皮质不同功能区微血管内的碱性磷酸酶表达随年龄的增加不断增加,成年时达到峰值;软脑膜血管进入皮质的方式分四种:T字形、Y字形、大弧形、小弧形;相同年龄不同部位的Lv、Sv和Vv显示运动皮质区、感觉皮质区、视觉皮质区、听觉皮质区、嗅觉皮质区呈现下降趋势,且运动皮质区和感觉皮质区与嗅觉皮质区比较Lv、Sv、Vv微血管密度有统计学意义(P<0.05)。老年组各功能区血管密度均较成年组降低,但无统计学意义(P>0.05)。结论 改良后的碱性磷酸酶染色法能清晰显示C57BL/6小鼠大脑皮质微血管构筑,将为脑血管疾病研究及动物模型制备提供形态学参考。

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    Abstract: Objective To observe and analyze the different functional areas of cerebral cortex in C57BL/6 mice of different months. Methods Improved alkaline phosphatase staining was used to reveal the microvascular morphology of the cerebral cortex of C57BL/6 mice, including motor cortex (primary motor cortex, secondary motor cortex), sensory cortex (primary somatosensory cortex, secondary somatosensory cortex), visual cortex (primary visual cortex, secondary visual cortex), auditory cortex (primary auditory cortex, secondary auditory cortex), olfactory cortex (extrorhinal cortex, entorhinal cortex). Images were captured by OLMPUS BX51 microscope combined with Image-Pro Plus 5.1 software. The microvascular length density (Lv), microvascular surface area density (Sv) and microvascular volume density (Vv) were analyzed by Image-Pro Plus 5.1 software. Results The levels of alkaline phosphatase in microvessels of different functional areas of cerebral cortex increased with age and reached the peak in adults. There were four patterns for pia vessels to enter the cortex including T shape, Y shape, large arc, small arc. The Lv, Sv and Vv in different functional areas of the same aged mice showed a downtrend trend in the motor cortex, sensory cortex, visual cortex, auditory cortex and olfactory cortex. Moreover, the microvascular density of Lv, Sv and Vv in the motor cortex and sensory cortex was statistically significant compared with those in the olfactory cortex (P<0.05). The vascular densities of different functional areas in the aged mice were lower than those in the adult ones, but there was no statistical significance (P>0.05). Conclusion The improved alkaline phosphatase staining may clearly reveal the microvascular architecture in cerebral cortex of C57BL/6 mice and it provides morphological reference for the research of cerebrovascular diseases and the preparation of animal models.

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  • 收稿日期:2023-02-22
  • 最后修改日期:2023-06-26
  • 录用日期:2023-12-04
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