京尼平苷通过PI3K/Akt通路促进糖尿病大鼠皮肤溃疡创面愈合
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江西科技学院

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基金项目:江西省教育厅课题(GJJ202016),江西省卫生健康委科技计划项目(202311153;20204112;20204111)。


Geniposide promotes skin ulcer wound healing in diabetic rats through PI3K/Akt pathway
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Jiangxi University

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    摘要:

    目的 探讨京尼平苷对糖尿病性皮肤溃疡的保护作用及其机制。方法 将大鼠分为正常组、模型组、京尼平苷亚组(Gen-L: 200 mg/kg; Gen-H: 500 mg/kg)。在糖尿病大鼠皮肤溃疡造模后,各组大鼠均灌胃生理盐水或京尼平苷(n = 6)。每天给药1次,每天记录各组伤口愈合情况及炎症情况。在糖尿病性皮肤溃疡治疗7天后,分别通过测量皮肤溃疡创口面积、组化切片与TUNEL染色及Western blot蛋白免疫印迹法量化分析创面愈合,细胞凋亡及其相关调节蛋白表达的变化情况。结果 与模型组相比,京尼平苷(200 mg/kg和500 mg/kg)口服能显著促进糖尿病大鼠创面愈合,增加损伤处的收缩。在糖尿病大鼠皮肤创面凋亡研究中,京尼平苷亚组TUNEL染色阳性细胞明显减少(P<0.05)。京尼平苷能显著抑制皮肤炎症,促进创面修复可能与促进PI3K和Akt蛋白磷酸化有关。结论 京尼平苷通过抑制炎症反应和细胞凋亡促进糖尿病大鼠皮肤创面修复。

    Abstract:

    Objective To investigate the protective effect of geniposide on diabetic skin ulcer and its mechanism. Methods Rats were divided into normal group, model group and geniposide subgroup (Gen-L: 200 mg/kg; Gen-H: 500 mg/kg). The diabetic rats were given normal saline or geniposide by intragastric administration (n = 6). Treatment was administered once a day, and the wound healing and inflammation of each group were recorded every day. After 7 days of treatment for diabetic skin ulcers, the wound area, tissue sections, TUNEL staining and Western blot were used to quantitatively analyze the changes of wound healing, apoptosis and related regulatory protein expression. Results Compared with the model group, geniposide (200 mg/kg and 500 mg/kg) orally could significantly promote the wound healing and increase the contraction of the injured area in diabetic rats. In the study of skin wound apoptosis in diabetic rats, TUNEL staining positive cells in geniposide subgroup were significantly reduced (P<0.05). Geniposide can significantly inhibit skin inflammation and promote wound repair, which may be related to the promotion of PI3K and Akt phosphorylation. Conclusion Geniposide can promote skin wound repair in diabetic rats by inhibiting inflammatory response and apoptosis.

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  • 收稿日期:2023-02-17
  • 最后修改日期:2023-06-13
  • 录用日期:2023-12-04
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