黄芩苷对注意缺陷多动障碍动物模型SHR大鼠核心症状影响研究
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1.河南中医药大学;2.河南中医药大学第一附属医院

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国家自然科学基金青年基金(82104928),中国博士后科学基金面上项目(2021M701123),河南省特色骨干学科课题(No.STG-ZYX06-202150),河南中医药大学仲景青年学者科研启动项目(CZ0168-04)


Study of core symptom impact on baicalein in an animal model of attention deficit hyperactivity disorder SHR rat
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1.Henan University of Traditional Chinese Medicine;2.The First Affiliated Hospital of Henan University of Traditional Chinese Medicine

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    摘要:

    目的 通过旷场和Morris水迷宫实验,探究黄芩苷调控注意缺陷多动障碍动物模型核心临床症状的疗效及其潜在机制。方法 将30只SHR大鼠随机分为模型组、盐酸哌甲酯(MPH)组、黄芩苷组、黄芩苷+丁苯那嗪组、MPH+丁苯那嗪组,每组6只,另设6只WKY大鼠为对照组。MPH组(1.5 mg/kg)、黄芩苷组(150 mg/kg)按体重(1 ml/100 g)灌胃给药,空白组、模型组给等量生理盐水,MPH+丁苯那嗪组、黄芩苷+丁苯那嗪组在灌胃给药同时,按体重(0.5 ml/100 g)腹腔注射丁苯那嗪(3 mg/kg)。连续给药4周。在规定时间进行旷场和水迷宫实验,记录并分析实结果。结果 MPH组和黄芩苷组在旷场实验中运动总距离和平均速度较模型组显著减小(P<0.05),在水迷宫实验中潜伏期较模型组显著缩短(P<0.05)、在目标象限运动距离和停留时间占比及穿越平台次数较模型组显著增加(P<0.05),且这两组间无明显差异;黄芩苷+丁苯那嗪组在旷场实验中运动总距离和平均速度较模型组显著减小(P<0.05),较黄芩苷组明显增大,在水迷宫实验中潜伏期较模型组显著缩短(P<0.05),较黄芩苷组显著延长(P<0.05)、在目标象限运动距离和停留时间占比较模型组明显增加,较黄芩苷组显著减少(P<0.05)。结论 黄芩苷能够改善SHR大鼠多动、冲动、注意力不集中等核心症状,其疗效的发挥可能与调控DA囊泡转运相关。

    Abstract:

    Objective To explore the efficacy and potential mechanism of baicalin in regulating the core clinical symptoms of ADHD through the Morris water maze test and The open field test. Methods Thirty SHR rats were randomly divided into model group, methylphenidate hydrochloride(MPH) group, baicalin group, baicalin + tetrabenazine group and MPH + tetrabenazine group, with 6 rats in each group. Another 6 WKY rats were used as normal control group. The rats in the MPH group (1.5 mg/kg) and the baicalin group(150 mg/kg) were given the corresponding drugs (1 mL/100 g) by gavage, and those in the normal control group and the model group were given an equal volume of normal saline by gavage . In addition to the corresponding drug gavage,the rats in the MPH+tetrabenazine group and the baicalin+tetrabenazine group were given intraperitoneal injection of tetrabenazine(3 mg/kg) according to body weight (0.5 ml/100 g).The course of treatment was 4 weeks for all groups. Open field and Morris water maze experiments were carried out at the specified time, and the experimental results were recorded and analyzed Results In the open field experiment, the total distance and average speed in the MPH group and baicalin group were significantly lower than those in the model group (P < 0.05), and there was no significant difference between the two groups (P > 0.05);In the open field experiment, the total distance and average speed in the baicalin + bubenazine group were significantly lower than those in the model group (P < 0.05), and obviously higher than those in the baicalin group. In the water maze test, the latency of baicalin + bubenazine group was significantly shorter than that in the model group (P < 0.05), and significantly longer than that in the baicalin group (P < 0.05). The percentage of movement distance and stay time in the target quadrant in the baicalin + bubenazine group were obviously higher than those in the model group. It was significantly lower than that in baicalin group (P < 0.05).Conclusion Baicalin can control the core symptoms of hyperactivity, impulse and inattention in SHR rats, and its curative effect may be related to the regulation of dopamine vesicle transport.

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  • 收稿日期:2023-01-05
  • 最后修改日期:2023-10-18
  • 录用日期:2023-11-03
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