IBS-D 大鼠肠道菌群-短链脂肪酸代谢轴变化特点及丁酸钠干预作用研究
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1.广州中医药大学东莞医院;2.广州中医药大学第二临床医学院;3.广州中医药大学第二临床医学院(广东省中医院)

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Alterations of Gut Microbiota - Short Chain Fatty Acid Axis in IBS-D Rats and the Effect of Sodium Butyrate
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1.Dongguan Hospital of Guangzhou University of Chinese Medicine;2.The Second Clinical College of Guangzhou University of Chinese Medicine

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    摘要:

    目的 探索 IBS-D 大鼠模型的肠道菌群-短链脂肪酸代谢轴变化特点,以及探讨丁酸钠对该轴的影响。方法 将 17 只Wistar大鼠随机分为 3 组,采用 4%醋酸灌肠联合束缚应激诱导 IBS-D 大鼠模型,造模期间予以丁酸钠腹腔注射,采用 16sRNA 技术检测大鼠粪便菌群,液相色谱-质谱联用(LC-MS)检测大鼠粪便短链脂肪酸含量。结果 与正常组比较,模型组体重下降,AWR 评分、粪便含水率明显升高,肠道益生菌相对丰度下降,布劳特氏菌属(Blautia)相对丰度明显升高,粪便乙酸含量显著增加,丁酸、戊酸含量明显下降;与模型组比较,丁酸钠组大鼠体重增加,AWR 评分、粪便含水率明显降低,布劳特氏菌属(Blautia)相对丰度显著下降,而粪便丁酸、戊酸含量显著升高。结论 丁酸钠可通过改善肠道菌群-短链脂肪酸代谢轴失调缓解 IBS-D 大鼠腹泻及结肠内脏高敏。

    Abstract:

    Objective To explore the alterations of gut microbiota - short chain fatty acid metabolic axis in IBS-D rat model, and to explore the effect of sodium butyrate on this axis. Methods Seventeen Wistar rats were randomly divided into three groups. The IBS-D rat model was induced by 4% acetic acid enema combined with restraint stress. During the modeling period, sodium butyrate was injected intraperitoneally. The fecal flora of rats was detected by 16sRNA technology, and the fecal short chain fatty acid content of rats was detected by liquid chromatography mass spectrometry (LC-MS). Results Compared with the normal group, the weight of the model group was decreased, AWR score and fecal water content have a significant increase, the relative abundance of intestinal probiotics was decreased,while that of g-Blautia was elevated, the fecal acetic acid content was increased significantly, and the contents of butyric acid and valeric acid was decreased significantly in the model group. After drug intervention, compared with the model group, the weight of rats in the sodium butyrate group was increased, the AWR score and fecal water content was decreased significantly, the relative abundance of g-Blautia was decreased significantly, and the fecal butyric acid and valeric acid content was obviously increased. Conclusions Sodium butyrate can alleviate diarrhea and colon visceral hypersensitivity in IBS-D rats by improving the imbalance of gut microbiota - short chain fatty acid metabolic axis.

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  • 收稿日期:2022-12-16
  • 最后修改日期:2023-03-02
  • 录用日期:2023-09-05
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