Objective To investigate the therapeutic effect and mechanism of transcutaneous electrical acupoint stimulation ( TEAS) combined with human chorionic gonadotrophin ( hCG) on blastocyst implantation dysfunction in rats. Methods Female rats were divided randomly into control, model, and treatment (TEAS+hCG)groups (n= 6 per group). Rats in the treatment group received TEAS+ hCG injections for 3 consecutive days starting from the late diestrus phase. On the day of proestrus, females were co-housed with male rats at a 2 ∶ 1 ratio. On gestation day 3, rats in the model and treatment groups received a subcutaneous injection of mifepristone suspension (5 mg / kg) to establish a blastocyst implantation dysfunction model. On gestation day 9, implanted blastocyst numbers, endometrial morphology, and expression levels of estrogen receptor α ( ERα), progesterone receptor (PR), and leukemia inhibitory factor ( LIF) were evaluated. Results The number of implanted blastocysts was significantly lower in the model group compared with the control group (p= 0. 005). The number was significantly increased after treatment with TEAS + hCG ( p= 0. 027) compared with the model group. The endometrium was significantly thinner in the model group compared with the control group, with underdeveloped pinopodes. TEAS+hCG restored the endometrial thickness to a similar level to the control group, and improved the development of pinopodes.Expression levels of ERα mRNA ( p= 0. 028), ERα protein ( p= 0. 012), and PR protein ( p= 0. 014) were significantly lower in the model group compared with the control group. Treatment with TEAS+hCG resulted in upward trends in the protein expression levels of ERα, PR, and LIF. Conclusions TEAS+hCG treatment may regulate endometrial expression of ERα, PR, and LIF, promote endometrial and pinopode development, improve endometrial receptivity, and effectively treat blastocyst implantation dysfunction in rats.