Skin modeling of transdermal drug delivery system refers to experimental models that mimic the structure and function of human skin to explore and evaluate absorption, penetration, and efficacy of medicines in transdermal drug delivery. It provides an alternative to traditional human skin experiments and reduces the use of human skin in medical research, which is convenient, controllable, and cost effective. For skin models of transdermal drug delivery systems, this article introduces commonly used animal skin models, artificial skin models, and recombinant human skin models from the perspective of the transdermal absorption pathway of medicines, and analyzes their advantages,disadvantages, and applications so provide references the research and development of transdermal formulations and topical therapies.