Objective The present situation and hotspots of KM mouse research were visualized by CiteSpace software, which provided a reference for breaking through the bottleneck of KM mouse research and finding new ideas in China. Methods A total of 3981 articles were retrieved from CNKI and Web of Science databases from January 1, 2004 to June 10, 2023, of which the number of publications, countries ( regions), institutions, author cooperation network,keyword co-occurrence, clustering, burst words, and cited English literature for analysis. Results The number of documents published in Chinese was decreased and the number of documents published in English was increased gradually in KM mouse research. The three countries with the most published articles were China, the United States, and Japan,where the leading research institutions were the China Agricultural University, Chinese Academy of Sciences, and Nanjing University of Chinese Medicine. The main authors at home and abroad were Liu Weihui, Li Guangwu, and Zhao Xin. The domestic research focused on the toxicology, immunology, and reproduction of KM mouse, and the foreign research focused on the cell biology and physiology of KM mouse. Conclusions KM mouse have been used and popularized in our country for more than 70 years, mainly focusing on toxicology, immunology, and reproduction. Although the use of KM mouse has led tove made allowed some achievements in experiments, certain it is necessary to solve their problems in the future, such as the standardization of population breeding, the discovery of the dominant research fields, and the further promotion of animal ethics, should be resolved.