针刺损伤后修复中的淋巴样结构
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作者单位:

1. 山东中医药高等专科学校解剖教研室,山东 烟台 264100; 2. 山东中医药高等专科学校口腔教研室,山东 烟台 264100

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R-33

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Lymphoid structure in repair of spinal cord acupuncture injury
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Affiliation:

1.Department of Anatomy, Shandong College of Traditional Chinese Medicine, Yantai 264100, China; 2. Department of Stomatology, Shandong College of Traditional Chinese Medicine, Yantai 264100, China

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    摘要:

    目的 观察正常及损伤后小鼠脊髓中淋巴管的分布及特点,探讨脊髓损伤修复过程中有无淋巴管的参与。 方法 成年雄性 KM 小鼠 39 只,小鼠随机分为两组:正常组 6 只和损伤组 33 只。 将损伤组小鼠随机分为术后 1 d 组、术后 3 d 组、术后 5 d 组、术后 7 d 组和术后 14 d 组,每组 6 只。 损伤组部分小鼠针刺损伤后死亡 3 只,后随机补充样本。 正常组不损伤脊髓,损伤组采用针刺法制备脊髓损伤。 通过免疫组织化学方法检测脊髓中淋巴管内皮细胞的分布,观察正常及针刺损伤后小鼠脊髓中淋巴管内皮细胞标记物同源异型盒基因转录因子 1( prox1)、淋巴管内皮细胞透明质酸受体-1(lyve-1)、平足蛋白(podoplanin)以及血管内皮细胞标记物 CD34 的表达情况。通过对脊髓样本进行免疫荧光染色,观察 lvye-1/ prox-1、lyve-1/ podoplanin 及 CD34 / prox-1 的共表达情况,探讨新生淋巴管内皮细胞的来源。 结果 正常成年小鼠脊髓中存在淋巴管样结构,并呈节段性分布,横向走行于白质与灰质间;针刺损伤处的脊髓出现新生淋巴管样结构,同时表达 prox-1、podoplanin、lyve-1 和 CD34;脊髓损伤处瘢痕化后,新生的淋巴管样结构消失。 结论 正常成年小鼠脊髓中存在节段性横向分布的淋巴管样结构;脊髓损伤处重建过程中有新生淋巴管样结构的参与,新生淋巴管内皮细胞可能来源于周围既存的淋巴管或血管内皮细胞。

    Abstract:

    Objective To observe the distribution and characteristics of lymphatic vessels in normal and injured mouse spinal cord, and to determine if lymphatic vessels participate in the repair of spinal cord injury. Methods Thirtynine adult male KM mice were divided randomly into a normal group (n = 6) and an injured group (n = 33). Mice in the injured group were further divided randomly into mice examined on postoperative days 1, 3, 5, 7, and 14. Three mice in the injury group died after acupuncture injury, and the sample was subsequently supplemented randomly. Spinal cord damage was induced in the injured group by acupuncture, while mice in the normal group had no spinal cord damage. The distribution of lymphatic endothelial cells (LECs) in the spinal cord was detected by immunohistochemistry, and expression levels of the lymphatic endothelial cell markers prospero-related homobox-1 ( prox-1), lymphatic vessel endothelial cell hyaluronic acid receptor-1 ( lyve-1), and flat foot protein ( podoplanin), and the vascular endothelial cell marker CD34were observed in the spinal cords in normal and acupuncture-injured mice. Spinal cord samples were examined by immunofluorescence staining, and the source of new LECs was explored by observing the co-expression of lyve-1/ prox-1,lyve-1/ podoplanin, and CD34 / prox-1. Results Lymphangioid structures were present in the spinal cord in normal mice and were distributed in segments, laterally between the white matter and gray matter. Nascent lymphangioid-like structures appeared in the spinal cord at the site of acupuncture injury, and prox-1, podoplanin, lyve-1, and CD34 were expressed simultaneously; however, these nascent lymphangioid-like structures disappeared after scarring during spinal cord injury. Conclusions Segmental, transversely distributed lymphangioid-like structures are present in the spinal cord in normal adult mice, and neonatal lymphangioid-like structures are involved in the reconstruction of spinal cord injury. These nascent LECs may originate from the surrounding existing lymphatic vessels or from vascular endothelial cells.

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陈丹丹,于宁,刘然,孟繁伟.针刺损伤后修复中的淋巴样结构[J].中国比较医学杂志,2024,34(01):65~72.

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  • 收稿日期:2023-02-17
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  • 在线发布日期: 2024-03-04
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