肉豆蔻曲代替麸煨肉豆蔻组成方新二神丸 的止泻作用研究
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1. 河南中医药大学,郑州 450046;2. 河南省中药特色炮制技术工程研究中心,郑州 450046; 3. 国家中医药管理局中药炮制传承基地,郑州 450046

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R-33

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Study on the antidiarrheal effect of the new “Ershen pills” composed of nutmeg koji instead of bran stewed nutmeg
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1. Henan University of Chinese Medicine, Zhengzhou 450046, China; 2. Henan TCM Characteristic Processing Technology Engineering Research Center, Zhengzhou 450046, China; 3. National Administration of Traditional Chinese Medicine TCM Processing and Inheritance Base, Zhengzhou 450046, China

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    摘要:

    目的 基于脾肾阳虚泄泻小鼠模型探讨肉豆蔻曲配伍组成新二神丸的止泻作用。 方法 将 KM 小鼠随机分为正常组、模型组、二神丸Ⅰ组(盐补骨脂 + 麸煨肉豆蔻)、二神丸Ⅱ组(盐补骨脂 + 肉豆蔻曲)、二神丸Ⅲ组(盐补骨脂 + 肉豆蔻生品)、盐补骨脂组、肉豆蔻曲组,采用“氢化可的松 + 番泻叶”的复合造模方法建立脾肾阳虚泄泻小鼠模型,观察各组小鼠一般体征、各脏器病理变化,检测小鼠各脏器指数、小肠推进率、胃残留率、血清胃动素(MTL)、胃泌素(GAS)、肾上腺酮(CORT)、促甲状腺激素(TSH)、睾酮(T)、肿瘤坏死因子 α(TNF-α)、白细胞介素 1β(IL-1β)水平,对粪便微生物进行 16S rDNA 测序及信息分析。 结果 造模后,与正常组相比,模型组小鼠体重减轻,活动量减少,小肠推进率显著升高,胃残留率、各脏器指数均显著降低,血清 GAS、CORT、TSH、T 水平均显著下降,MTL、TNF-α、IL-β 水平均显著上升,肠道菌群物种多样性降低;给药后,各给药组小鼠上述指标和症状均有不同程度改善,且二神丸Ⅱ组整体上优于二神丸Ⅰ组、二神丸Ⅲ组、盐补骨脂组及肉豆蔻曲组。 结论 肉豆蔻曲与盐补骨脂配伍使用对脾肾阳虚泄泻的作用效果显著,肉豆蔻发酵后能减少其毒性风险,增强其温脾止泻作用,为肉豆蔻曲的开发奠定基础。

    Abstract:

    Objective Exploring the antidiarrhea effect of new Ershen pills composed of nutmeg koji based on the spleen and kidney yang deficiency diarrhea mouse model. Methods KM mice were randomly divided into normal, model,Ershen pills Ⅰ ( salt psoralen + bran-stewed nutmeg), Ershen pills Ⅱ ( salt psoralen + nutmeg koji), Ershen pills Ⅲ(salt psoralen + nutmeg raw product), salt psoralen, and nutmeg koji groups. The combined modeling method of hydrocortisone + senna leaf was used to establish the diarrhea mouse model with spleen-kidney yang deficiency. General signs and pathological changes of each organ were observed. Various organ indexes, the small intestine propulsion rate,gastric residual rate, serum motilin ( MTL), gastrin ( GAS), adrenal ketone ( CORT), thyroid stimulating hormone (TSH), testosterone ( T), tumor necrosis factor-α ( TNF-α), and interleukin 1β ( IL-1β) were assessed. 16S rDNA sequencing and data analysis were conducted for fecal microorganisms. Results After modeling, compared with the normal group, the weight and activity of mice in the model group were reduced, the small intestine propulsion rate was significantly increased, the gastric residual rate and organ indexes were significantly decreased, serum GAS, CORT, TSH, and T levels were significantly decreased, MTL, TNF-α, and IL-1β levels were significantly increased, and intestinal flora species diversity was decreased. After administration, the above indexes and symptoms were improved by various degrees in each administration group, and the Ershen pills Ⅱ group was better than Ershen pills Ⅰ, Ershen pills Ⅲ, salt-psoralea, and nutmeg koji groups. Conclusions Combined use of nutmeg koji and salt psoralen has a remarkable effect on diarrhea of spleen-kidney yang deficiency. Fermented nutmeg reduces its toxicity risk and enhances its effect of warming the spleen and preventing diarrhea, which facilitates the development of nutmeg koji.

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范蒙蒙,张雨,李红伟,张振凌,李凯.肉豆蔻曲代替麸煨肉豆蔻组成方新二神丸 的止泻作用研究[J].中国比较医学杂志,2024,34(01):48~57.

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  • 收稿日期:2023-06-14
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  • 在线发布日期: 2024-03-04
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自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
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