小鼠甲状腺功能减退模型的建立
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1. 四川省自贡市第一人民医院甲乳外科, 四川 自贡 643000;2. 西南医科大学附属医院甲状腺外科, 四川 泸州 646000;3. 西南医科大学附属医院临床医学研究中心, 四川 泸州 646000

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R-33

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Establishment of hypothyroidism model in mice
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1.Department of Thyroid and Breast Surgery, First People’s Hospital of Zigong City of Sichuan Province, Zigong 643000, China; 2. Department of Thyroid Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 3. Clinical Medical Research Center, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China

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    摘要:

    目的 将 C57BL/ 6 和 KM 小鼠甲状腺通过两种不同术式全切后,验证甲状腺功能减退模型造模是否成功,同时比较不同术式造模的成功率差异。 方法 本研究采用 C57BL/ 6 和 KM 小鼠进行结扎法(术式Ⅰ)和止血法(术式Ⅱ)进行甲状腺全切操作,并记录操作详细过程,利用酶联免疫吸附法检测、对比术前及术后小鼠的血清TT3、TT4 及 TSH 水平、体重及颈部组织苏木精-伊红(HE)染色验证模型。 结果 两种术式均造成两种属模型组小鼠血清 TT3、TT4 降低(P< 0. 05),TSH 升高(P< 0. 001)。 术式Ⅰ组和术式Ⅱ组小鼠术后 28 d 内存活率分别为C57BL/ 6 小鼠:40%和 60%;KM 小鼠:50%和 40%。 两种属手术组小鼠术后体重较假手术组均明显升高。 HE 染色和显微镜观察=结果表明,两种属小鼠颈部切取组织为甲状腺组织,且离体后甲状腺后背膜完整。 结论 两种手术方式均能造成两种属小鼠甲状腺功能减退,但需要熟悉小鼠甲状腺及周围组织解剖关系,提高操作熟练度,预防术后低钙及感染从而提高造模后小鼠存活率。

    Abstract:

    Objective Total thyroidectomy of C57BL/ 6 and KM mice was performed by two different surgical Methods to verify the success of mouse hypothyroidism model modeling, and compared the success rate of different surgical Methods. Methods C57BL/ 6 and KM mice underwent total thyroidectomy by ligation ( operation method Ⅰ) or hemostasis (operation method Ⅱ), and the detailed operation processes were recorded. Serum TT3, TT4 and TSH levels detected by enzyme-linked immunosorbent assay, body weight, and hematoxylin-eosin ( HE)-stained neck tissue were compared before and after surgery to verify the model. Results Serum TT3 and TT4 levels were decreased (P< 0. 05) and TSH was increased (P< 0. 001) in both model groups. The 28-day postoperative survival rates were 40% and 60% in groups Ⅰ and Ⅱ, respectively, and 50% and 40% in KM mice. Body weights were significantly higher in both model groups compared with the sham control group. HE staining and microscopic observation showed that the cervical tissue in both strains was thyroid tissue, and the back membrane of the thyroid remained intact after isolation. Conclusions Both surgical method can induce hypothyroidism in C57BL/ 6 and KM mice; however, it is necessary to consider the anatomical relationship of the thyroid gland to the surrounding tissue, improve the proficiency of the surgical operation, prevent the occurrence of postoperative hypocalcemia and infection, and thus improve the survival rate of the model mice.

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兰浩淼,张立,毛雨,谢林浚,车红缨.小鼠甲状腺功能减退模型的建立[J].中国比较医学杂志,2024,34(01):40~48.

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  • 收稿日期:2023-04-06
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  • 在线发布日期: 2024-03-04
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