芩蓟凉血合剂对痔病相近动物模型的影响
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上海中医药大学附属市中医医院肛肠科,上海 200071

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R-33

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Effect of Qinji Liangxue mixture on animal models of hemorrhoids
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Department of Anorectal Surgery, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China

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    摘要:

    目的 探讨芩蓟凉血合剂(Qinji Liangxue mixture,QLM)对抗痔作用的机制。 方法 分别使用巴豆油制剂(croton oil preparation,COP)、冰醋酸制备大鼠急性痔病动物模型,将 80 只 SD 雌性大鼠,随机分为 COP 组与冰醋酸组两组,每组 40 只。 COP 组,随机分为对照组 1(CA 组),模型组 1(CB 组),QLM 低剂量组 1-1(CC 组),QLM 高剂量组 1-2(CD 组),地奥司明组 1(CE 组),每组 8 只;冰醋酸组,随机分为对照组 2(GA 组),模型组 2(GB组),QLM 低剂量组 2-1(GC 组),QLM 高剂量组 2-2(GD 组),地奥司明组 2(GE 组),每组 8 只。 除对照组,其余组均使用 COP 或冰醋酸处理,随后使用不同药物治疗 7 d。 使用苏木精-伊红(HE)染色观察 COP 及冰醋酸诱导的肛门直肠组织形态学,使用 ELISA 检测 COP 诱导的大鼠血清 IL-6、TNF-α 水平,记录冰醋酸诱导的大鼠肛周溃疡面积。 将 24 只 C57BL/ 6 小鼠随机分为对照组(A 组),QLM 低剂量组(B 组),QLM 高剂量组(C 组),肾上腺色腙片组(D 组),每组 6 只,连续治疗 7 d,使用毛细管法及断尾法评价 QLM 的止血疗效。 结果 QLM 能够显著改善痔病相近动物模型的病理损伤,减轻 COP 诱导的病变程度评分(P< 0. 05),降低血清 IL-6、TNF-α 水平(P< 0. 05),减少冰醋酸诱导的肛周溃疡面积(P< 0. 05),缩短凝血及出血时间(P< 0. 05)。 结论 QLM 具有良好的抗痔活性,其作用可能是通过抗炎、止血、减少组织损伤实现的。

    Abstract:

    Objective To explore the mechanism underlying the anti-hemorrhoid effects of Qingji Liangxue mixture(QLM). Methods Models of acute hemorrhoid in rats were established using a croton oil preparation (COP) and glacial acetic acid. A total of 80 SD female rats were randomly divided into two major groups: the COP group and the glacial acetic acid group, each consisting of 40 rats. In the COP group, rats were further randomly assigned to the following subgroups: control group 1 (group CA), model group 1 ( group CB), low-dose QLM group 1-1 ( group CC), high-dose QLM group 1-2 (group CD), and diosmin group 1 (group CE), with eight rats in each subgroup. In the glacial acetic acid group, rats were randomly divided into the following subgroups: control group 2 (group GA), model group 2 (group GB ),low-dose QLM group 2-1 (group GC), high-dose QLM group 2-2 ( group GD), and diosmin group 2 ( group GE), with eight rats in each subgroup. Except for the control groups, all other subgroups were treated with COP or glacial acetic acid then treated with various drugs for 7 days. HE staining was used to observe anorectal histomorphology induced by COP and glacial acetic acid. ELISAs were used to measure serum levels of IL-6 and TNF-α induced by COP. Additionally, the area of perianal ulcers induced by glacial acetic acid was recorded. Twenty-four C57 mice were randomly divided into a control group (group A), low-dose QLM group ( group B), high-dose QLM group ( group C), and adrenal chromazone tablet group (group D). Results QLM significantly improved the pathological injury of animal models with similar hemorrhoids,reduced the score of pathological changes induced by COP (P< 0. 05), reduced the serum levels of IL-6 and TNF-α (P<0. 05), reduced the area of perianal ulcers induced by glacial acetic acid ( P< 0. 05), and shortened the time of coagulation and bleeding (P< 0. 05). Conclusions QLM has a good anti-hemorrhoid activity, which may be achieved by anti-inflammatory effects, hemostasis, and reducing tissue damage.

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周蒙恩,李鹏,金文琪,王若琳,郭修田.芩蓟凉血合剂对痔病相近动物模型的影响[J].中国比较医学杂志,2024,34(01):33~39.

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  • 收稿日期:2023-08-28
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  • 在线发布日期: 2024-03-04
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自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
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