BAPN 诱导小鼠胸主动脉夹层合并急性肺损伤 模型的建立
作者:
作者单位:

1. 宁夏医科大学临床医学院,银川 750004;2. 空军军医大学西京医院心血管外科,西安 710032; 3. 宁夏医科大学总医院心脏大血管外科,银川 750004

作者简介:

通讯作者:

中图分类号:

R-33

基金项目:


Establishment of a mouse model of thoracic aortic dissection with acute lung injury by BAPN
Author:
Affiliation:

1.Clinical College of Ningxia Medical University, Yinchuan 750004, China; 2. Department of Cardiovascular Surgery, Xijing Hospital, Air Force Medical University, Xi’an 710032, China; 3. Department of Cardiovascular Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 采用 β-氨基丙腈(β-aminopropionitrile monofumarate,BAPN)1 g / ( kg·d)饮水给药的方式,构建一种可行性高、稳定的胸主动脉夹层(thoracic aortic dissection,TAD)合并急性肺损伤(acute lung injury,ALI)的小鼠模型,为研究 TAD 合并 ALI 提供合理的动物模型。 方法 选取 45 只 SPF 级 3 周龄 C57BL/ 6J 雄性小鼠,随机分为 CON 组 15 只(正常饮食水)和 BAPN 组 30 只(与无菌水配置成 1 g / (kg·d)的溶液饮水给药),持续 4 周。 实验期间,观察两组小鼠一般情况、成模率,通过测量小鼠胸主动脉最大直径和主动脉组织苏木素-伊红(HE)染色,验证小鼠 TAD 模型并将 BAPN 组分为 TAD 组和 Non-TAD 组。 进一步检测 CON 组、Non-TAD 组和 TAD 组小鼠肺组织 HE 病理染色、湿干重比(dry / wet weight ratio,W/ D)及肺泡灌洗液(broncho alveolar lavage fluid,BALF)中总蛋白水平和白细胞介素-1β ( interleukin-1β, IL-1β )、 白介素-6 ( interleukin-6, IL-6 )、肿瘤坏死因子-α ( tumor necrosis factor-α, TNF-α)表达情况验证小鼠 TAD 合并 ALI 模型。 结果 BAPN 干预明显延缓小鼠体重和饮水量的增加。 与 CON 组和 Non-TAD 组相比,TAD 组小鼠胸主动脉最大直径明显增粗(P< 0. 05);主动脉 HE 染色显示主动脉中层明显增厚,主动脉壁结构破坏、紊乱;肺组织 HE 染色显示肺间质明显水肿及炎性渗出,伴肺泡腔扩大,肺泡壁上皮脱落及透明膜形成,肺损伤病理评分显著增加(P< 0. 05);肺组织 W/ D、BALF 中总蛋白水平及 IL-1β、IL6、TNF-α 表达也明显升高(P< 0. 05),而另外两组上述指标无明显差异。 结论 通过 BAPN 饮水给药的方式,可成功建立胸主动脉夹层合并急性肺损伤的小鼠模型。

    Abstract:

    Objective A feasible and stable mouse model of thoracic aortic dissection ( TAD) combined with acute lung injury (ALI) was established using β-aminopropionitrile monofumarate (BAPN) 1 g / ( kg·d) administered in drinking water. The mouse model of TAD combined with acute lung injury (ALI) was established to provide a rational animal model to study TAD combined with ALI. Methods Forty-five SPF-grade 3-week-old C57BL/ 6J male mice were selected and randomly allocated to a CON group (normal dietary water; 15 mice) or BAPN group (administration in sterile water at 1 g / (kg·d); 30 mice) for 4 weeks. During the experimental period, the general condition and modeling rate of mice were observed. TAD model mice were validated, and the BAPN group was divided into TAD and non-TAD groups by measuring the maximum diameter of the thoracic aorta and HE staining of aortic tissues. HE pathological staining, the wet /dry weight (W/ D) ratio, total protein level in bronchioalveolar lavage fluid (BALF), and interleukin(IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) in BALF) were used to validate the TAD combined ALI model in mice. Results BAPN treatment significantly delayed the increase in body mass and water intake of mice. Compared with CON and non-TAD groups, the maximum diameter of the thoracic aorta of mice in the TAD group was significantly thickened (P< 0. 05). HE staining of the aorta showed significant thickening of the middle aortic layer, and the structure of the aortic wall was damaged and disordered. HE staining of lung tissues showed significant interstitial edema and inflammatory exudation accompanied by enlargement of alveolar lumen, alveolar wall epithelial exfoliation and hyaline membrane formation, and a significant increase in the pathological scores of lung injury (P< 0. 05). Total protein levels and expression of IL-1β,IL-6, and TNF-α in lung tissue, W/ D ratio, and BALF were also significantly increased ( P< 0. 05), whereas no significant difference was observed in the above indexes between the other two groups. Conclusions A mouse model of thoracic aortic dissection combined with acute lung injury can be established by BAPN administration in drinking water.

    参考文献
    相似文献
    引证文献
引用本文

买志妍,江丽青,朱翰朝,张溧昀,王云,段维勋. BAPN 诱导小鼠胸主动脉夹层合并急性肺损伤 模型的建立[J].中国比较医学杂志,2024,34(01):9~16.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-08-02
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2024-03-04
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭