糖尿病大鼠心肌纤维化与心肌AGEs/RAGE水平的变化研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

河南省教育厅科学技术研究重点项目(项目编号14B890010)


The Gradual Changes of Myocardial Fibrosis and AGEs/RAGE Levels of Diabetic Rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    【摘要】目的 通过构建Ⅱ型糖尿病大鼠模型,研究该模型心肌纤维化和心肌AGEs/RAGE水平的变化情况。方法 雄性SD大鼠高脂饲料饲喂6周后,过夜空腹12h,腹腔注射小剂量链脲佐菌素(STZ,30mg/kg)。注射3天和7天后检测大鼠随机血糖浓度,两次随机血糖≥16.7mmol/L为Ⅱ型糖尿病大鼠建模成功。在建模成功后第4、8和12周,检测大鼠血FBG、Insulin和左心室AGEs、Hyp含量及Masson染色、RAGE表达的变化。结果 造模成功后第4、8和12周,糖尿病对照组(4D,8D,12D)血FBG水平均较同周次正常对照组(4C,8C,12C)显著升高,体重显著下降;造模成功后第12周时,糖尿病对照组(12D)大鼠Insulin显著高于同周次正常对照组(12C)和4周时糖尿病对照组(4D);从造模成功后第8周开始,糖尿病对照组(8D,12D)心肌Hyp、CVF、AGEs和 RAGE与正常对照组(8C,12C)相比均显著升高;2型糖尿病大鼠心肌AGEs/RAGE水平与CVF呈显著正相关。结论 高脂饮食饲喂加腹腔注射小剂量STZ,能够成功复制Ⅱ型糖尿病大鼠模型。随着病程的延长,心肌纤维化和心肌AGEs含量、RAGE蛋白表达均呈逐渐上升趋势,且Ⅱ型糖尿病大鼠心肌AGEs/RAGE水平与心肌纤维化存在有正相关关系。

    Abstract:

    【ABSTRACT】 Objective In order to investigate the gradual changes of myocardial fibrosis and myocardial AGEs/RAGE levels of type Ⅱ diabetes, the authors established the rats model for type Ⅱ diabetes. Methods Male SD rats werefed high-fat fodder for 6 weeks, and then they were injected a small dose of streptozotocin (STZ, 30mg/kg) after overnight fasting 12h. The random blood GLU was detected in the 3th and 7th day respectively after STZ injection. When the rats of which the random blood GLU concentration was greater than or equal to 16.7 mmol/L in two times were successful rats’ model of type Ⅱ diabetes. Six rats of group C and six rats of group D with fasting 12h were killed after intraperitoneal anesthesia at the 4th, 8th and 12th week after the type Ⅱ diabetic rats successfully established. The blood was used for FBG, insulin detection. A portion of the left ventricle was used for Masson staining and immunohistochemical detection of RAGE, and the other portion of the left ventricle was used for the content detection of AGEs and Hyp. Results The FBG level of group D were significantly higher than them of group C at the same week. The body weight of group D were significantly lower than them of group C at the same week. Blood insulin of group D at the 12th week were significantly higher than those of group C at the 12th week and group D at the 4th week. Myocardial Hyp, CVF, AGEs and RAGE of group D were significantly higher than those of group C at the 8th, 12th week. The AGEs/RAGE level of myocardium in type Ⅱ diabetic rats was positively correlated with CVF. Conclusion The model of type Ⅱ diabetes were successfully established through intraperitoneal injecting a low-dose streptozotocin to SD rats that had been fed high-fat fodder. The rats of type Ⅱ diabetes demonstrated myocardial fibrosis at the 12th week after the diabetic rats’ model successfully established. With the further development of type Ⅱ diabetes, myocardial fibrosis, the content of myocardial AGEs, RAGE protein expression of the diabetic rats had an upward trend. The level of AGEs/RAGE was positively correlated with the degree of myocardial fibrosis of type Ⅱ diabetic rats.

    参考文献
    相似文献
    引证文献
引用本文

侯改霞.糖尿病大鼠心肌纤维化与心肌AGEs/RAGE水平的变化研究[J].中国实验动物学报,,().

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2018-02-20
  • 最后修改日期:2018-04-09
  • 录用日期:2018-05-02
  • 在线发布日期: 2018-11-12
  • 出版日期:
征稿啦 |“科技伦理前沿谈”征文通知
关闭