Establishment and evaluation of a mouse model of type II diabetes mellitus complicated by tuberculosis
Received:July 16, 2013  Revised:August 07, 2013
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KeyWord:Tuberculosis; Type II diabetes mellitus; Concurrent infection; Mouse model
           
AuthorInstitution
刘芳 上海市公共卫生临床中心
杨华 上海市公共卫生临床中心
冯艳玲 上海市公共卫生临床中心
周文江 上海市公共卫生临床中心
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Abstract:
      Objective To establish a mouse model of type II diabetes mellitus complicated by tuberculosis and make a evaluation to the process of lesions in lung tissue. Methods Feed the mice with high-fat and high-sugar, inject intraperitoneally STZ to C57BL/6J mice, create the type II diabetes mellitus mouse model. Then, infected with Mycobacterium tuberculosis H37Rv by nasal drip, to establish a mouse model of type II diabetes mellitus complicated by tuberculosis. Dissect the mice separately at 1-8th, 10th and 12th week after infection, make visual observation to the lesions of lung, colony counting and pathological examination, HE and acid-fast staining. Results The visual observation results showed that the lung infection has deteriorated as the infection time increased. The focus of the infection reached to 80% of the whole lung at the 8th week, and the scope of lesions began to shrink at the 10th week. The typical granulomatous consolidation appeared in lung tissue after 8 weeks of the infection, and till the 10th week, the part of the lesions of lung tissue began to repair. The mycobacterium tuberculosis in the lungs were revealed by acid-fast stain. Conclusions Compare with the tuberculosis mouse model in gross inspection, colony counting, pathological changes, the type II diabetes mellitus complicated by tuberculosis mouse model is characterized with early onset , fast disease progression, and similar to the patients with clinical performance. Therefore, the experiment proves that the establishment of the type II diabetes mellitus complicated by tuberculosis mouse model is successful.
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