【Abstract】 Objective: Explore the method and evaluation of establishment of the stress-induced rats of intestinal dysfunction. Methods: Sprague-Dawley rats were assigned to two groups randomly: the normal group and the model group . The model group was treated by using chronic astricting, excess fatigue and out of constant diet .It would continue for three weeks. At the last day of the third week, the content of GAS, IL-2, in serum, MTL, SS, ACTH, in plasma were measured by the method of ELISA kit. Results: Compared with the normal group, the model group had many different and apparent changes of appearance and action, their weight and the mass of their livers and spleens are much less than the normal group, the level of these biomarkers are reducing apparently. Conclusion: Stress-induced rats of intestinal dysfunction can be successfully established by using chronic astricting, excess fatigue and out of constant diet in the SD rats, and the biomarkers such as GAS,IL-2,ACTH,MTL,SS can be used to evaluation of the model.