[Abstract] Objective To establish drug screening model with primary cultured prostatic epithelial cells from rats in vitro. Methods The ventral prostate was freed from adult SD rat under sterile conditions and weighted, then cut into 1mm3 slices, after being digested with collagenase 2 for 1 h, the prostate cells were harvested through filtering and centrifuging. All cells were seeded into 24-well plate and cultured for cell morphology and immunohistochemistry detection. The prostatic epithelial cells were seeded respectively into 96-well plate and 24-well plate, treated with epristeride （0.1-1000µM）and tamoxifen （0.1-1000µM）for 72 h after being cultured for 12 d. The prostatic epithelial cell survival rate was detected by CCK-8 method, and the IC50 value of epristeride and tamoxifen was calculated, then further observed their effects on the growth and cell morphology of prostatic epithelial cells. Results According to cell morphology and immunohistochemical detection result, all cells from rat prostate displayed typical epithelial cell shape, and their expressions of cytokeratin antibody and prostate specific antigen were positive, such results suggested that the harvested cells were prostatic epithelial cell. After the prostatic epithelial cell being treated with epristeride for 72 h, its growth significantly been inhibited, and the IC50 value of epristeride was 42.7µM. Cell morphology results further showed that epristeride didn’t significantly changed the shape of prostatic epithelial cell, but it could reduce the survival prostatic epithelial cells number. While tamoxifen didn’t inhibit the cell growth, and had no obvious effect on cell number and cell shape. Conclusion Benign prostatic hyperplasia drug screening model could be successfully establish with primary cultured prostatic epithelial cells from rats in vitro.