The effects of overexpressed mice UNCV protein on apoptosis in Hela cells
Received:April 15, 2013  Revised:May 22, 2013
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KeyWord:UNCV; apoptosis; serum starvation; doxorubicin
                             
AuthorInstitution
刘冰 军事医学科学院实验动物中心
徐嫄 军事医学科学院实验动物中心
赵爽 军事医学科学院实验动物中心
王冬平 军事医学科学院实验动物中心
杨蕾蕾 军事医学科学院放射与辐射医学研究所
李微 军事医学科学院实验动物中心
尚世臣 军事医学科学院实验动物中心
张广州 军事医学科学院实验动物中心
李文龙 军事医学科学院实验动物中心
曾林 军事医学科学院实验动物中心
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Abstract:
      Objective To study the effects of the mice UNCV protein on Hela cells apoptosis. Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into Hela cells. The Hela cells which stably overexpressed UNCV protein were selected. The effects of overexpressed UNCV protein on apoptosis of Hela cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry. Results The Hela cells which stably overexpressed UNCV protein were obtained. The results of cell counting and flow cytometry showed that overexpression of the UNCV protein inhibited apoptosis of Hela cells induced by serum starvation. The effects of UNCV protein on apoptosis of Hela cells induced by doxorubicin were not significantly different. Conclusions Overexpression of UNCV protein inhibited apoptosis of Hela cells induced by serum starvation.
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