[Abstract] Objective To studies on Correlation between chemical media sensitivity of guinea pig tracheal Smooth Muscle and phenotype of feature. Methods Select 36 guinea pigs in according to difference of features which includes coat color, eye color and gender. By phenotype the animals are divided into 6 groups, white coat color and black eye and female-group (WBEF), white coat color and black eye and male group (WBEM), white coat color and red eye and female group (WREF), white coat color and red eye and male group(WREM), variegated coat color and black eye and female group(VBEF), variegated coat color and black eye and male group(VBEM), only 6 animals in each group. By method of spiral cutting with knife blade to prepare isolated Guinea pig trachea spiral strip, which is incubated in Kreb's liquid for 2 hours tension balance, Trachea spiral strip contraction is caused while histamine concentrations in bath is 2.0x10-3g•L-1 or acetylcholine concentrations in bath is 2.0x10-4g•L-1. Using BL420 biological signal acquisition system and tension sensor for measuring tension variation values, which is applied for analysis correlation between contraction of tracheal spiral strip induced by histamine or acetylcholine and difference of guinea pig’eye color, coat color, gender. SPSS11.5 soft ware is used to analyzing Data in a=0.05 reliability. Results Guinea Pig coat color and eye color phenotype have significant impact on the chemical medium sensitivity of the tracheal smooth muscle, P＜0.05,diffence has statistic significance, Phenotype of white coat color is higher than phenotype of mixed coat color and phenotype of red eye is higher than phenotype of black eye on chemical medium sensitivity of guinea pig tracheal smooth muscle. Phenotype of gender has no significant effect on chemical medium sensitivity. Conclusion Tracheal smooth muscle sensitivity to chemical media and phenotype of coat color or eye color have significant correlation, phenotype of gender has no significant correlation. Animal Models of asthma should be preferred to white eye phenotype of guinea pigs.