Reproduction of a mouse model of burn with infection
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    Abstract:

    【Abstract】 Objective To reproduce a simple mice model of burn with infection for the study of prevention and treatment of infected wound. Method Self-made wood burn board and boiling water was used to reproduce burn injury ( with diameter of 8 mm) on both sides of the back in 30 Balb/C mice for different duration (5s, 10s, 15s with 10 mice each). 48 h post bum, the wounds were used for histological observation to determine the depth of injury. Deep II degree scald on the back in another 72 mice was made. Immediately after bum,20 ul of liquid containing 1?06, l?07 and 1?08 CFU/mL Staphylococcus aureus ATCC 25923 was respectively inoculated to the wounds (with 24 rats for each amount) with scraping method. Inflammatory reaction of wounds was examined with HE staining on 72 h post inoculation day for screening the best inoculation concentration. On 3, 7 and 14 day post inoculation day,the number of subeschar bacteria was respectively counted.at last, the best condition was chose to establish animal model. The time of wound healing was recorded and pathological examination with HE staining was observed at the 7th and 14th day after injuring to determine whether the wound infection model establishment successfully. Result Bum for 10 s was identified as injury time resulting in Deep II degree scald according to histological. l?08 CFU/ml was chose as best inoculation concentration. The bacteria isolated from wounds was identified as Staphylococcus aureus.The subeschar bacteria count within 14 day post injury was was more thanl?05 CFU/g of tissue. Compared with the control group, infected group showed a longer time (from 15.92?.34 d to 21?.95 d) of wound healing (P<0.01).Obvious inflammatory cell infiltration was observed with poor patient prognosis. Conclusion In mice,the wound infection model was establised successfully as a reliable model for the study of infection of burn wound.

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History
  • Received:December 31,2012
  • Revised:January 20,2013
  • Adopted:January 28,2013
  • Online: July 04,2013
  • Published: