The Change of Tissue and Serum Cytokine Levels in Experimental Acaques Diabetes Model
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the National High Technology Research and Development Program of China (863 Program)

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    Abstract:

    Objective To investigate the change of cytokine(IL-6,IL-10, TNF-α) in the pathogenesis of acaques diabetes model.Methods we used STZ (30 mg/kg of body weight, intravenously,3days)to five macaques. The dynamic changes of IL-6, IL-10, TNF-α in serum were measured for 19 months.The animal was sacrificed when impending death. The expression of IL-6,IL-10, TNF-α in pancreas islet, liver and kidney were observed with immunohistochemistry staining. Image analysis and statistical analysis were carried out of all the result above. Results Compared with the contents before injection IL-10 increased significantly (P<0.01)while IL-6, TNF-α reduced (P<0.05,P<0.01).The quantity of IL-6 and TNF-α were significantly decreased in pancreas islet than that in control group(P<0.05). The quantity of IL-10 was significantly increased in pancreas islet than that in control group(P<0.01). The immunohistochemical reaction of TNF-α in liver significantly decreased comparedwith control group (P<0.05). Concusion The level of cytokine in experimental acaques diabetes model is mimic that of human diabetes and may be useful in assessing safety and efficacy of protective agents.

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History
  • Received:December 21,2012
  • Revised:February 26,2013
  • Adopted:March 01,2013
  • Online: July 04,2013
  • Published: