Objective Comparative analysis of left and right ventricular function of cTnTR141W transgenic mice of dilated cardiomyopathy, studying whether cTnTR141W transgenic mice can be used as a right ventricular cardiomyopathy animal model. Methods A Comparative study of cardiac tissue left and right ventricular in vivo structural and functional changes between non-transgenic mice (NTG) and cTnTR141W transgenic mice at 2, 4, 6 and 8 months of age by 7.0T high-field magnetic resonance imaging technology. Changes of left and right ventricular end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) were analyzed quantitatively with ages. Meanwhile, pathologic changes of myocardial tissue from NTG and cTnTR141W transgenic mice were analyzed at 6 month of age. Results Compared with NTG, left and right ventricular volume of cTnTR141W transgenic mice showed an increased tendency, and ejection fraction showed a trend of decrease. Right ventricular ejection fraction showed earlier significant difference between NTG and cTnTR141W transgenic mice (P< 0.05). Compared to NTG, structure and function pathophysiological changes of right ventricular and left ventricular of cTnTR141W transgenic mice trend to be serious with aging at the same time. Dilated cardiomyopathy phenotype was showed in right and left ventricular after 4 month of age typically. Conclusion Dilated cardiomyopathy phenotype of cTnTR141W transgenic mice left ventricular and right ventricular occurred at the same time. This mice can be used as animal models, which research on right ventricular function in cardiomyopathy.