Study on Pathology and Gene Expression in Microtus fortis Infected with Schistosoma japonicum
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    Abstract:

    Objective To study on pathology and gene expressions in Microtus fortis infected with Schistosoma japonicum, which provide reference for the further researches on the mechanisms of anti-schistosomiasis of M. fortis. Methods M. fortis was challenged with 2000cercariae of S. japonicum. Lungs and livers of M. fortis were collected at 6, 10, 15, 20 and 30 days after infection. Histological changes of livers and lungs of all voles were observed by light microscopy. The expression of Lyz,RT1-Db1,Cd74,C1qa, Thra and Igf1 genes were detected by real-time PCR. Result 6-10 days after infected with S. japonicum, massive hemorrhage was observed in lungs, hepatic cells showed vacuolar degeneration, liver sinusoidal expanded, a large numbers of eosinophils and a small amount of other inflammatory cells such as neutrophils, macrophages exuded around vascular of lungs and livers, which were gradually recovered from twentieth day. Lyz,RT1-Db1and Cd74 genes were significantly up-regulated in lungs and livers from sixth day after infection and gradually restored from twentieth day;C1qa gene was significantly up-regulated in lungs, Thra gene was significantly down-regulated in lungs, Igf1 gene was down-regulated in livers. Conclusion Eosinophil may play an important role in anti-schistosomiasis of M. fortis and up-regulation or down-regulation of some genes may also have suitable function.

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History
  • Received:November 06,2012
  • Revised:November 20,2012
  • Adopted:December 10,2012
  • Online: April 18,2013
  • Published: