Objective To explore the changes and roles of inflammatory factors in Acute Lung Injury Inducde by Swine Influenza virus H9N2 in Mice. Methods The experimental mice were infected with swine influenza virus (H9N2) by nose dropping. To obtain lung tissue homogenate of mice in 2, 4, 6, 10 and 14 day after infection, then concentration of TNF-α, IL-1β, IL-6 and IL-10 were detected. Results Concentration of TNF-α,IL-1β,IL-6 and IL-10 in lung tissue homogenate of infected group were significantly higher than control group in different time point (P< 0.05)，TNF-α and IL-1β were tended towards normal, but IL-6 and IL-10 were still in the high levels after 14 days. Conclusion Inflammation factor plays a major role in acute lung injury induced by H9N2 subtype swine influenza virus in mice, TNF-α and IL-1β were displayed a proinflammatory effect, and IL-10 was likely the same as IL-6 that were play an anti-inflammatory effect.