Establishment and evaluation on the Model of Focal Cerebral Ischemia/ Reperfusion induced by monofialment in Kunming Mice with different body weight
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    Abstract:

    Aim: To establish the mouse focal cerebral ischemia/reperfusion model with suture method ,and study the stability of the model of middle cerebral artery (MCAO). Method : One hundred Kunming mice , weighing 20-40g, half male and half female, were randomly divided into 10 groups according to the body weight: sham group (♂), sham group (♀), group A(♂,20-25g), group B (♂,25-30g), group C (♂,30-35g),group D (♂,35-40g),group E (♀,20-25g), group F (♀,25-30g), group G (♀,30-35g), group H (♀,35-40g).10 mice in each group. Nylon thread (Φ0.128-0.180mm) was plugged into the internal carotid artery in mouse to occlude the right middle cerebral artery reversibly. The reliability of the MCAO model was evaluated by the degree of neurological deficit score and TTC stain. Result:Other groups except sham groups all emerged the change in nervous behavioral function and clear infarction area in TTC after cerebral ischemic reperfusion. There were significant increases of behavior scores and infarct volume in group A and group C (P < 0.01), compared with group C and group D. The neural deficit scores in group E and group F were markedly higher than that in group G and group H (p<0.01). Meanwhile, the volume of cerebral infarction enhanced significantly (P<0.05) in group E and group F in comparison with group G and group H. Compared with female mice,the success rates of MCAO model were increased in male mice while the mortality rates were decreased. The neurological scores in group A were observably higher than that in group E (p<0.01). In addition, there were significant increases of behavior scores and infarct volume in group B(P < 0.01), compared with group F. Conclusion : The mouse focal cerebral ischemic/reperfusion model with suture method is stable and simple. There are many crucial factors to guarantee the success of mouse focal cerebral ischemic/reperfusion model induced by nylon monofilament, such as the mice weight matching the diameter of monofilament, the depth of line, sex selection, operation time, and suitable temperature during experiment. Experimental investigation shows that weighing about 25g Kunming male mice is fit for the studies of focal cerebral ischemic/reperfusion.

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History
  • Received:October 08,2012
  • Revised:December 04,2012
  • Adopted:December 11,2012
  • Online: April 18,2013
  • Published: