Objective By cloning and analyzing the partial sequence of Cricetulus griseus 16s ，the purpose of this research is to qust for framework and function. Methods According to conservative domain of the published sequence of 16s gene of rodent animal in GenBank，a pair of primers that can amplify Cricetulus griseus 16s gene was designed and synthesized. The sequence was compared with published 16s genes in GenBank by Blastn，based on 16s sequences the molecular phylogenetic trees were constructed by neighbor-joining method，unweighted pair-group method with arithmetic means and taxonomic status of cricetulus griseus was estimated at molecular level. Results The part sequences of 16s gene in cricetulus griseus were obtained. The results showed that Cricetulus griseus, Mesocricetus auratus and Cricetus cricetus had the closest phylogenetic relationship，Microtus fortis, Microtus kikuchii and Microtus rossiaemeridionalis had the closest phylogenetic relationship，Mus and Rattus had the closest phylogenetic relationship. Conclusion Cricetulus griseus and Mesocricetus auratus had closest relationship. The evolution tree built from the homology of 16S genes of Cricetulus griseus and other 7 kinds of animal was consistent with the traditional taxonomic results.