Objective To establish the Escherichia coli O127 infected animal model and establish the real-time PCR method for detecting TGF-β1. Methods The inflammatory bowel mice model was constructed by intragastrical administration of Escherichia coli O127. The TGF-β1 of mice colon tissue was analyzed using chimeric fluorescence quantitative PCR. Results The inflammatory bowel of mice was established successfully. The expression level of TGF-β1 of experimental group was higher than control group. Conclusions TGF-β1 can be taken as an evaluation standard for inflammatory bowel animal model.