Objective To compare the different role of 5 behavioral tests in amotrophic lateral sclerosis transgenic mouse Methods This study was divided into model group (SOD1-G93A mice) and negative control group (littermates neg ative control). Behavior changes were evaluated by 5 behavioral assessments (Assessment of general condition, weighing, rotarod test, grip strength measurement and step length analysis). Results (1) Assessment of general condition: The general condition of the model group began to decline at day 89. In day 101, the differences between negative control group and model group were significant for the first time, P=0.000. (2) Weighing: Weighing of the model group began to decline at week 15 (day 105), and the differences between negative control group and model group were significant for the first time, P=0.026. (3) Rotarod test: The time of rotarod test began to decline at week 11(day 77). In week 13 (day 91), the differences between negative control group and model group were significant for the first time, P=0.047. (4) Grip strength measurement: Grip strength for hindlimb began to decline at week 10 (day 70). In week 13 (day 91), the differences between negative control group and model group were significant for the first time, P=0.000. (5) Step length analysis: Step length for hindlimb began to decline at week 14 (day 98). In week 15 (day 105), the differences between negative control group and model group were significant for the first time, P=0.000. Conclusion Grip strength measurement is better than others in behavioral tests of the SOD1-G93A mice.