Objective To investigate regulation of serum cyclic nucleotides (cAMP and cGMP) and the repair of dopamine neurons in mice with Yin deficiency and moving wind syndrome of Parkinson’ s disease ( PD) by Compound Dihuang Granule. Methods Overall, 24 male C57BL/ 6 mice were randomly divided into normal ( Normal), model (MPTP + NS), and treatment (MPTP + CDG) groups with eight mice in each group. The normal group was injected with normal saline and the model group was intraperitoneally injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish the PD animal model. Both groups were injected once a day for 5 consecutive days. The treatment group was administered Compound Dihuang Granule (CDG) by gavage once a day for 7 days. The TCM syndrome attribute of the model was verified by etiology, symptoms, the objective index, and formula test in PD mice and the effect of CDG on expression of cyclic nucleotides and tyrosine hydroxylase in the serum of PD mice with Yin deficiency and moving wind syndrome was assessed. Results Significant differences in behavior, the tongue chroma value ratio, serum cyclic nucleotide content, and tyrosine hydroxylase content were found between model and normal control groups (P< 0.05). CDG treatment significantly improved the behavior and related objective indexes of mice (P< 0.05). Conclusions The mouse model of Yin deficiency and moving wind syndrome of Parkinson’ s disease prepared by intraperitoneal injection of MPTP is stable and reliable. CDG regulates the content of cyclic nucleotides in serum and effectively repairs damaged dopaminergic neurons.