Objective To quickly evaluate the immune enhancement effect of Chinese herbal compound extract (CHCe) and study the potential mechanism using in vitro cell and in vivo zebrafish models. Methods In the in vitro experiments, RAW 264. 7 mouse mononuclear macrophage cells were treated with vinorelbine tartrate, and the protective effect of CHCe on vinorelbine tartrate-induced toxicity was evaluated. The zebrafish immunocompromised model was established using vinorelbine tartrate. The number of macrophages and their phagocytic function, the number of T- lymphocytes, and the expression of inflammation- and immune-related genes in zebrafish were used to observe the effects of CHCe in the zebrafish immunocompromised model. Results The result showed that CHCe had good biosafety and increased the survival rate of RAW 264. 7 cells treated with 250 μg / mL vinorelbine tartrate. In vivo studies showed that 222, 667 and 2000 μg / mL CHCe significantly inhibited the reduction of macrophages induced by vinorelbine and enhanced the phagocytic ability of macrophages. Both 222 and 667 μg / mL CHCe significantly increased the number of T- lymphocytes, and 222, 667 and 2000 μg / mL CHCe significantly restored the down-regulation of rag1 and rag2 genes in the model group, and down-regulated the expression of tumor necrosis factor-α. A dose of 222 μg / mL CHCe up-regulated the relative expression level of interleukin-12, and 2000 μg / mL CHCe up-regulated the relative expression level of interferon-γ. Conclusions In vivo and in vitro experiments showed that CHCe had a marked immunity enhancement effect.