Analysis of the effects of two reoviruses on the intestinal flora of mice based on 16S rRNA sequencing
Received:March 01, 2021  
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DOI:10. 3969 / j.issn.1005-4847. 2022. 01. 013
KeyWord:reovirus; 16S rRNA; intestinal flora; infection way
王诗雨 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
林家锋 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
蒋欣如 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
孙淼 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
王颖 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
陶晓莉 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州
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       Objective To explore the effects of two reoviruses, T1L and NBV, on the structure of mouse intestinal flora. Methods The mice were randomly divided into five groups: control, instillation-NBV ( int-NBV), oral- NBV, instillation-T1L ( int-T1L ), and oral-T1L groups, with five mice in each group. The control group was intragastrically administered with phosphate buffered saline, and other groups were each infected with a viral titer of 2×107 PFU/ mL. After 7 days, mouse feces were collected and three samples were selected using the heavier stool weights from each group. After V3+V4 amplification, 16S rRNA technology was used to estimate the richness, diversity and composition of bacterial flora. Results After T1L and NBV instillation, the abundance and diversity of intestinal flora were lowered compared with the control group, with a significant decrease (P<0. 05) in the int-T1L group. The abundance and diversity were markedly augmented (P<0. 05) in the int-NBV group compared with the oral-NBV group. At the phylum level, the abundance of Firmicutes was markedly reduced in both the oral-T1L and oral-NBV groups compared with the control groups, and the abundance of Bacteroidetes was markedly reduced in both the int-T1L and int-NBV groups compared with the control group. At the genus level, Romboutsia was markedly lower in the int-T1L, oral-T1L and oral-NBV groups versus the control group, and Alistipes was markedly augmented in the int-T1L group (P< 0. 05). Conclusions Two reoviruses, T1L and NBV, reduced the abundance and diversity of mouse flora and may destroy flora balance through the reduction of beneficial bacteria or the increase of pathogenic bacteria. Different infection routes have different effects on the flora of mice.
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