Establishment and genetic characteristics of the Genetic Diversity mice resource
Received:March 11, 2021  
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DOI:10. 3969 / j.issn.1005-4847. 2021. 04. 007
KeyWord:genetic diversity; complex traits; whole-exon sequencing; inbred mice; outbred mice
                    
AuthorInstitution
王欣佩 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
张伶燕 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
劳兰兰 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
叶凡 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
刘云鹏 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
李丽 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
秦川 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,卫健委人类疾病比较医学重点实验室, 北京市人类重大疾病实验动物模型工程技术研究中心,北京
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Abstract:
       Objective We aimed to establish a novel resource consisting of genetically diverse mice and perform a preliminary characterization of the genetic traits of these mice. Methods (1) A multi-parent panel was used to create the Genetic Diversity mice through appropriate crosses. ( 2 ) Whole-exon sequencing was used to analyze the genetic characteristics of the established Genetic Diversity outbred and inbred mice. ( 3) Using the genome sequences of the founders and offspring, we created the chromosome physical maps of these mice and calculated the founder contributions. Results (1) Genetic Diversity inbred mice were characterized by stable inheritance and consistent phenotype and were suitable for rapid phenotypic correlation analysis. (2) Compared with the inbred mice, the Genetic Diversity outbred mice showed superior growth and reproductive performance, and more genetic variation, which led to a “ mosaic-like ” chromosomal pattern. As unique individuals, the outbred mice are ideal for the study of subtle genetic regulatory mechanisms. (3) The founder contributions to the progeny lines were not uniform and genetic preference existed among the offspring. Conclusions Compared with the more conventional inbred mice, the Genetic Diversity mice established by breeding from multiple lines possessed diverse genetic variations. The Genetic Diversity outbred and inbred lines are associated with pros and cons but complement one another, and together represent a novel and powerful tool for the study of complex genetic traits.
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