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| Study of the immune responses mediated by specific CD8+T cells to influenza A virus H1N1 in the lungs of young and aged C57BL/ 6 mice |
| Received:June 15, 2021 |
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| DOI：10. 3969 / j.issn.1005-4847. 2021. 04. 006 |
| KeyWord:influenza A virus; virus-specific CD8+T cells; immune response |
| Author | Institution |
| 刘洋 |
复旦大学附属公共卫生临床中心,上海 |
| 王超 |
复旦大学附属公共卫生临床中心,上海 |
| 任晓楠 |
复旦大学附属公共卫生临床中心,上海 |
| 李顺 |
复旦大学附属公共卫生临床中心,上海 |
| 秦波音 |
复旦大学附属公共卫生临床中心,上海 |
| 杨华 |
复旦大学附属公共卫生临床中心,上海 |
| 周晓辉 |
复旦大学附属公共卫生临床中心,上海 |
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| Abstract: |
| Objective To compare the immune response of specific CD8+ T cells induced by H1N1 Influenza A virus PR8 strain in the lungs of young and aged C57BL/ 6 mice. Methods Young ( 3 months) and aged ( 24 months) mice were intranasally infected with 490 plaque-forming units of PR8, then 8 days later, they were euthanized and their lungs collected. Fluorescence-activated cell sorting was used to compare the number and functions of viral antigen-specific CD8+T cells between the young and aged mice. Surface staining for the influenza virus MHC-I tetramer was performed to count the antigen-specific CD8+T cells; and intracellular cytokine staining was used to quantify the cytokines secreted by CD8+ T cells in response to influenza-specific peptides, including tumor necrosis factor ( TNF-α ), interferon-γ, interleukin-2, and granzyme B, which are involved in killing by CD8+ T cells.Results The proportion of virus-specific CD8+T cells in the aged mice was significantly lower than in the young mice. Moreover, the expression of interferon-γ, TNF-α, and interleukin-2 in activated CD8+T cells from the aged mice was significantly lower than in the young mice. In addition, the level of granzyme B expression on CD8+T cells in the aged mice was significantly lower. Conclusions The number of specific CD8+T cells induced by PR8 influenza virus was lower, and their function was impaired in the lungs of aged C57BL/ 6 mice compared with young mice. This implies that the response by specific CD8+T cells in the lungs of mice is impaired by aging. |
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