Study of the immune responses mediated by specific CD8+T cells to influenza A virus H1N1 in the lungs of young and aged C57BL/ 6 mice
Received:June 15, 2021  
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DOI:10. 3969 / j.issn.1005-4847. 2021. 04. 006
KeyWord:influenza A virus; virus-specific CD8+T cells; immune response
刘洋 复旦大学附属公共卫生临床中心,上海
王超 复旦大学附属公共卫生临床中心,上海
任晓楠 复旦大学附属公共卫生临床中心,上海
李顺 复旦大学附属公共卫生临床中心,上海
秦波音 复旦大学附属公共卫生临床中心,上海
杨华 复旦大学附属公共卫生临床中心,上海
周晓辉 复旦大学附属公共卫生临床中心,上海
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       Objective To compare the immune response of specific CD8+ T cells induced by H1N1 Influenza A virus PR8 strain in the lungs of young and aged C57BL/ 6 mice. Methods Young ( 3 months) and aged ( 24 months) mice were intranasally infected with 490 plaque-forming units of PR8, then 8 days later, they were euthanized and their lungs collected. Fluorescence-activated cell sorting was used to compare the number and functions of viral antigen-specific CD8+T cells between the young and aged mice. Surface staining for the influenza virus MHC-I tetramer was performed to count the antigen-specific CD8+T cells; and intracellular cytokine staining was used to quantify the cytokines secreted by CD8+ T cells in response to influenza-specific peptides, including tumor necrosis factor ( TNF-α ), interferon-γ, interleukin-2, and granzyme B, which are involved in killing by CD8+ T cells.Results The proportion of virus-specific CD8+T cells in the aged mice was significantly lower than in the young mice. Moreover, the expression of interferon-γ, TNF-α, and interleukin-2 in activated CD8+T cells from the aged mice was significantly lower than in the young mice. In addition, the level of granzyme B expression on CD8+T cells in the aged mice was significantly lower. Conclusions The number of specific CD8+T cells induced by PR8 influenza virus was lower, and their function was impaired in the lungs of aged C57BL/ 6 mice compared with young mice. This implies that the response by specific CD8+T cells in the lungs of mice is impaired by aging.
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