Discussion on aseptic tests of germ-free animals
Received:July 02, 2020  
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DOI:10. 3969 / j.issn.1005-4847. 2021. 01. 016
KeyWord:Germ-free animal; aseptic test; bacterial culture; 16S rRNA; PCR
李永军 浙江大学医学院附属第一医院传染病诊治国家重点实验室,国家感染性疾病临床医学研究中心, 感染性疾病诊治协同创新中心,杭州
李兰娟 浙江大学医学院附属第一医院传染病诊治国家重点实验室,国家感染性疾病临床医学研究中心, 感染性疾病诊治协同创新中心,杭州
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      In recent years, the role of intestinal microorganisms in human health and diseases has been widely researched. The application of a germ-free animal model in intestinal microecology is extensive and this model provides a very effective research tool for human physiological and pathological research. To ensure the quality of experimental animals, aseptic tests must be frequently performed. However, sterility tests are affected by many factors. At present, the traditional detection method are mainly bacterial and fungal culture and Gram staining. With the development of high- throughput sequencing, polymerase chain reaction method have been used to identify bacteria using 16S rRNA. However, these method have some limitations. In this paper, we discuss the advantages and disadvantages of these method and the influencing factors.
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